Protein kinase C lambda/iota (PKClambda/iota): a PKC isotype essential for the development of multicellular organisms.
Protein kinase C lambda/iota (PKClambda/iota): a PKC isotype essential for the development of multicellular organisms.
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发表时间：
2003
影响因子：
2.7
通讯作者：
A. Suzuki;K. Akimoto;S. Ohno
中科院分区：
文献类型：
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作者：
A. Suzuki;K. Akimoto;S. Ohno
PKClambda/IOTA属于PKC家族的第三类，非典型PKC(APKC)，由于其序列与传统的和新的PKC的序列不同，不仅在N端调节域，而且在激酶域也被观察到。虽然aPKC最明显的特征之一是其单一的、不重复的半胱氨酸富含结构域，但最近的研究表明，APKC的N端调控结构域具有额外的aPKC特有的结构基序，参与各种蛋白质-蛋白质相互作用，这对aPKC的调控和亚细胞靶向非常重要。APKC特异性结合蛋白的鉴定有助于从分子水平上理解aPKC的激活机制和生理功能。特别是，秀丽线虫蛋白的哺乳动物同源物PAR-3和PAR-6与aPKC结合的发现意外地为探索aPKC的一个迄今完全未知的关键功能开辟了一条新的途径，即作为进化保守的细胞极性机制的组成部分。随着基因组计划和模式生物遗传分析的巨大进步，这些进展将我们带入一个PKC研究的新时代，在这个时代，PKClambda/IOTA和Zeta之间的功能差异可以得到详细的讨论。
PKClambda/iota belongs to the third group of the PKC family, atypical PKC (aPKC), together with PKCzeta based on its sequence divergence from conventional and novel PKCs observed not only in the N-terminal regulatory domain but also in the kinase domain. Although one of the most distinct features of aPKC is its single, unrepeated cysteine-rich domain, recent studies have revealed that the N-terminal regulatory domain has additional aPKC-specific structural motifs involved in various protein-protein interactions, which are important for the regulation and the subcellular targeting of aPKC. The identification of aPKC-specific binding proteins has significantly facilitated our understanding of the activation mechanism as well as the physiological function of aPKC at the molecular level. In particular, the finding that the mammalian homologs of the Caenorhabditis elegans proteins, PAR-3 and PAR-6, bind aPKC unexpectedly opens a new avenue for exploring a thus far completely unrecognized critical function of aPKC, that is, as a component of an evolutionarily conserved cell polarity machinery. Together with the great progress in the genome project as well as in the genetic analysis of model organisms, these advances are leading us into the new era of aPKC study in which functional divergence between PKClambda/iota and zeta can be discussed in elaborately.