API LISTERIA, A NEW AND PROMISING ONE-DAY SYSTEM TO IDENTIFY LISTERIA ISOLATES
API LISTERIA, A NEW AND PROMISING ONE-DAY SYSTEM TO IDENTIFY LISTERIA ISOLATES
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DOI：
10.1128/aem.58.6.1857-1860.1992
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发表时间：
1992-06-01
影响因子：
4.4
通讯作者：
ROCOURT, J
中科院分区：
文献类型：
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作者：
BILLE, J;CATIMEL, B;ROCOURT, J
API 李斯特菌是一种新的 10 次测试条，用于对李斯特菌分离株进行 24 小时生化鉴定。通过这个商业系统，646 个李斯特菌菌株（包括本研究选择的非典型分离株）中的 85% 在物种和亚种水平上得到了识别，无需进行补充测试。一项新的测试将单核细胞增生李斯特菌与无害李斯特菌区分开来，因为前者不存在芳基酰胺酶。利用该系统，97.7%（258 株中的 252 株）所测试的单核细胞增生李斯特菌菌株被正确识别，并与 99.4%（176 株中的 175 株）所测试的无害利斯特氏菌菌株区分开来。除李斯特菌属以外的革兰氏阳性细菌。给出了完全不同的生化模式。该系统大大减少了传统识别所需的时间，因为结果可在 18 至 24 小时内获得。
API Listeria is a new 10-test strip for 24-h biochemical identification of Listeria isolates. With this commercial system, 85% of 646 Listeria strains, including atypical isolates selected for this study, were recognized at the species and subspecies level without a complementary test. A new test differentiates Listeria monocytogenes from L. innocua on the basis of the absence of arylamidase from the former. With this system, 97.7% (252 of 258) of the L. monocytogenes strains tested were correctly identified and differentiated from 99.4% (175 of 176) of the L. innocua strains also tested. Gram-positive bacteria other than Listeria spp. gave quite different biochemical patterns. This system considerably reduced the time needed for conventional identification, since results were available within 18 to 24 h.