A novel glucose sensor based on immobilization of glucose oxidase on the chitosan-coated Fe3O4 nanoparticles and the luminol-H2O2-gold nanoparticle chemiluminescence detection system
A novel glucose sensor based on immobilization of glucose oxidase on the chitosan-coated Fe3O4 nanoparticles and the luminol-H2O2-gold nanoparticle chemiluminescence detection system
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DOI：
10.1016/j.snb.2015.09.125
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发表时间：
2016-02-01
影响因子：
8.4
通讯作者：
Ehsani, Mahjoobeh
中科院分区：
文献类型：
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作者：
Chaichi, Mohammad Javad;Ehsani, Mahjoobeh
将葡萄糖氧化酶（GOX）有效地固定在Fe 3 O 4-壳聚糖（Fe 3 O 4-CS）纳米粒子载体上，构建了一种基于酶促H2 O2化学发光（CL）检测的新型高效葡萄糖生物传感器。以戊二醛（GA）为交联剂对酶进行共价固定化。(H2O2通过在戊二醛官能化的玻璃细胞中共价固定葡萄糖氧化酶来构建）。除了催化鲁米诺CL反应外，金纳米颗粒还对葡萄糖氧化酶和葡萄糖之间的酶促反应产生过氧化氢提供优异的催化活性。通过响应面分析法（RSM）确定了葡萄糖氧化酶的最佳固定化条件：酶/载体比为0.7，固定化pH值为6，交联时间为75 min，戊二醛（GA）浓度为0.2%。采用XRD、FT-IR和SEM对Fe 3 O 4、Fe 3 O 4-壳聚糖和Au纳米粒子进行了表征。该方法的线性范围为1 × 10 ~（-4）~ 8.5 × 10 ~（-7）mol·L ~（-1）（R ~（-2）= 0.9956）。检出限为4.3 × 10 ~（-7）mol·L ~（-1）。相对标准偏差（RSD）
A novel and efficient glucose biosensor based on the chemiluminescence (CL) detection of enzymatically generated H2O2 was constructed by the effective immobilization of glucose oxidase (GOX) on the Fe3O4-chitosan (Fe3O4-CS) nanoparticles support. The covalent immobilization of enzyme was performed using glutheraldehyde (GA) as a cross-linking agent. (H2O2 was constructed by one covalent immobilization of glucose oxidase in glutaraldehyde-functionalized glass cell). In addition to catalyzes the luminol CL reaction, gold NPs offer excellent catalytic activity toward hydrogen peroxide generation as a result of enzymatic reaction between glucose oxidase and glucose. Based on response surface methodology (RSM), the optimal immobilization conditions for glucose oxidase were obtained as: enzyme to support ratio, 0.7; Immobilization pH, 6; cross-linking time, 75 min; and glutaraldehyde (GA) concentration, 0.2%. The characterization of Fe3O4, Fe3O4-chitosan and Au nanoparticles was analyzed by XRD, FT-IR and SEM. The linear range of this novel method is 1 x 10(-4) to 8.5 x 10(-7) mol L-1 (R-2 = 0.9956). The detection limit is 4.3 x 10(-7) mol L-1. The relative standard deviation (RSD)