Compositional equivalency of RNAi-mediated virus-resistant transgenic soybean and its nontransgenic counterpart.
Compositional equivalency of RNAi-mediated virus-resistant transgenic soybean and its nontransgenic counterpart.
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DOI：
10.1021/jf500859c
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发表时间：
2014-05
影响因子：
6.1
通讯作者：
Xiuchun Zhang;Pingjuan Zhao;Kunxin Wu;Yuliang Zhang;M. Peng;Zhi-xin Liu
中科院分区：
文献类型：
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作者：
Xiuchun Zhang;Pingjuan Zhao;Kunxin Wu;Yuliang Zhang;M. Peng;Zhi-xin Liu
RNA沉默或RNA干扰(RNAi)由双链RNA(DsRNA)触发，是一种进化上保守的过程，在许多真核生物中都很活跃。具有发夹结构的工程植物，其中病毒基因以反向重复(IR)排列，从而使植物对植物病毒感染具有抵抗力。然而，目前还没有关于IR的插入是否发生了生物学上的重要变化的报道，IR赋予转基因植物对病毒的抗性。在本研究中，通过应用实质等价性原则比较了通过插入3个短IRS获得的抗病毒转基因大豆种子的组成，其中每个短IRS包含来自一种病毒的特定的高度保守的序列，以确定IR插入是否发生了显著的不良生物学变化。结果表明，这些抗病毒大豆品系的营养成分和抗营养素含量与非转基因大豆品系基本相同，大部分营养成分和抗营养素含量的测定值都在其他商品大豆品系的报道范围内。这些结果表明，在RNAi介导的抗病毒转基因大豆中插入IRS没有发生重要的生物学变化。这些结果可为开发RNAi介导的转基因大豆品种或其他具有更广泛抗病毒谱的作物提供基础信息。
RNA silencing or RNA interference (RNAi), which is triggered by double-stranded RNA (dsRNA), is an evolutionarily conserved process that is active in a wide variety of eukaryotic organisms. Engineering plants with hairpin construct in which the viral gene is arranged in inverted repeats (IR) renders plants resistant to plant virus infection. However, there is no report on whether biologically important changes occurred by the insertion of IR, which confer transgenic plants virus resistance. In the present study, the compositions of virus-resistant transgenic soybean seeds developed by insertion of three short IRs, each containing the specific, highly conserved sequences derived from one virus, were compared with those of nontransgenic counterparts by applying the principle of substantial equivalence to determine whether significant undesirable biological changes occurred by IR insertion. The results revealed that the nutrient components as well as antinutrient contents of these virus-resistant soybean lines are substantially equivalent to those of the nontransgenic counterparts, and the majority of the measured amounts of nutritional components and antinutrient contents are well within the range of values reported for other commercial soybean lines. The results imply that no biologically important changes occurred by the insertion of IRs in the RNAi-mediated virus-resistant transgenic soybeans. The results can serve as baseline information for developing RNAi-mediated transgenic soybean cultivars or other crops with broader spectrum virus resistance.