Coordinated peptidoglycan synthases and hydrolases stabilize the bacterial cell wall.
Coordinated peptidoglycan synthases and hydrolases stabilize the bacterial cell wall.
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DOI：
10.1038/s41467-023-41082-3
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发表时间：
2023-09-02
影响因子：
16.6
通讯作者：
Nan, Beiyan
中科院分区：
文献类型：
--
作者：
Zhang, Huan;Venkatesan, Srutha;Ng, Emily;Nan, Beiyan
肽聚糖(PG)定义细胞形状，保护细菌免受渗透胁迫。PG的生长和完整性需要合成酶和水解酶之间的协调作用，合成酶插入新的PG链，水解酶产生开口允许插入。然而，它们之间的协调机制仍然难以捉摸。莫诺霉素抑制一种被称为A类青霉素结合蛋白(APBPs)的PG合成酶家族，尽管aPBPs对粘液球菌黄色杆菌中的杆状形态不是必需的，但它可以折叠杆状。在这里，我们证明了被抑制的PBP1a2，一种aPBP，加速了DacB，一种水解性PG多肽酶对细胞极的降解。莫诺霉素促进DacB与PG的结合，从而通过PBP1a2降低DacB的迁移率。相反，DacB还调节aPBPs的分布和动态。我们的发现阐明了莫诺霉素的作用，并表明破坏PG合成酶和水解酶之间的协调可能比消除单个酶更致命。细胞壁肽聚糖的完整性和维持性对于细菌的生长和细胞形状是必不可少的。在这里，作者展示了合酶和水解酶的协同作用如何决定细菌细胞壁的完整性。合成酶插入新的肽聚糖链，水解酶产生开口以允许插入。
Peptidoglycan (PG) defines cell shape and protects bacteria against osmotic stress. The growth and integrity of PG require coordinated actions between synthases that insert new PG strands and hydrolases that generate openings to allow the insertion. However, the mechanisms of their coordination remain elusive. Moenomycin that inhibits a family of PG synthases known as Class-A penicillin-binding proteins (aPBPs), collapses rod shape despite aPBPs being non-essential for rod-like morphology in the bacterium Myxococcus xanthus. Here, we demonstrate that inhibited PBP1a2, an aPBP, accelerates the degradation of cell poles by DacB, a hydrolytic PG peptidase. Moenomycin promotes the binding between DacB and PG and thus reduces the mobility of DacB through PBP1a2. Conversely, DacB also regulates the distribution and dynamics of aPBPs. Our findings clarify the action of moenomycin and suggest that disrupting the coordination between PG synthases and hydrolases could be more lethal than eliminating individual enzymes. The integrity and maintenance of cell-wall peptidoglycan is essential for growth and cell shape in bacteria. Here, the authors show how the coordinated actions of a synthase, which inserts new peptidoglycan strands, and a hydrolase, which generates openings to allow the insertion, determine the integrity of bacterial cell wall.
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DOI：
10.1007/978-1-4939-3676-2_2
发表时间：
2016-01-01
期刊：
BACTERIAL CELL WALL HOMEOSTASIS: METHODS AND PROTOCOLS
影响因子：
--
作者：
Alvarez, Laura;Hernandez, Sara B.;Cava, Felipe
通讯作者：
Cava, Felipe
DOI：
10.1073/pnas.1219982110
发表时间：
2013-04-16
影响因子：
11.1
作者：
Nan, Beiyan;Bandaria, Jigar N.;Zusman, David R.
通讯作者：
Zusman, David R.
影响因子：
28.3
作者：
Dion, Michael F.;Kapoor, Mrinal;Garner, Ethan C.
通讯作者：
Garner, Ethan C.
影响因子：
7.7
作者：
Hussain, Saman;Wivagg, Carl N;Garner, Ethan C
通讯作者：
Garner, Ethan C
影响因子：
3.2
作者：
McPherson, DC;Popham, DL
通讯作者：
Popham, DL