Increasing the efficiency of in-capillary electrophoretically mediated microanalysis reactions via rapid polarity switching
Increasing the efficiency of in-capillary electrophoretically mediated microanalysis reactions via rapid polarity switching
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DOI：
10.1021/ac048595u
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发表时间：
2005-04-15
影响因子：
7.4
通讯作者：
Strein, TG
中科院分区：
文献类型：
--
作者：
Sanders, BD;Slotcavage, RL;Strein, TG
我们在此报告了一种新方法，可提高涉及在线反应的基于 CE 的方法的灵敏度或速度。快速极性切换 (RPS) 是一种通过快速（1-5 秒）和顺序切换所施加的势场来在线混合两种反应物溶液的新颖方法。通过采用 RPS 方法以及肌酸酐和碱性苦味酸盐之间的化学反应模型，可以显着提高灵敏度（或缩短分析时间）。离子试剂的对流和电泳堆积的增加似乎都有助于表观反应速率的提高。当与肌酐 Jaffe 方法的在线化学相结合时，RPS 方法可以将临床血清样本分析所需浓度范围内的肌酐测定速度提高 3 倍。新方法还允许在不需要麻烦且有问题的增强灵敏度池的情况下进行分析。
We report herein a new approach to enhance the sensitivity or speed of CE-based methods that involve in-line reactions. Rapid polarity switching (RPS) is used as a novel means for in-line mixing of two reactant solutions via rapid (1-5 s) and sequential switching of the applied potential field. By employing the RPS approach with a model chemical reaction, that between creatinine and alkaline picrate, significant enhancement in sensitivity (or a decrease in analysis time) is realized. Both increased convection and electrophoretic stacking of the ionic reagent appear to contribute to the rise in apparent reaction rate. When coupled with in-line chemistry of the Jaffe method for creatinine, the RPS methodology allows for 3-fold faster determination of creatinine in the concentration range needed for the analysis of clinical blood serum specimens. The new approach also allows the analysis to be performed without the need for the cumbersome and problematic enhanced sensitivity cell.