N-cadherin-dependent cell-cell contacts promote human saphenous vein smooth muscle cell survival
N-cadherin-dependent cell-cell contacts promote human saphenous vein smooth muscle cell survival
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DOI：
10.1161/01.atv.0000163183.27658.4b
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发表时间：
2005-05-01
影响因子：
8.7
通讯作者：
George, SJ
中科院分区：
文献类型：
--
作者：
Koutsouki, E;Beeching, CA;George, SJ
目的：血管平滑肌细胞（VSMC）凋亡被认为与动脉粥样硬化斑块不稳定有关。钙粘蛋白介导钙依赖性嗜同性细胞-细胞接触。我们研究了n -钙粘蛋白在VSMC细胞凋亡中的作用。方法与结果：在琼脂糖包被的孔中培养人隐静脉VSMCs，使钙粘蛋白介导的聚集体形成。使用几种方法（每种方法n >= 3）检测钙粘蛋白破坏后的细胞死亡和凋亡。从培养基中去除钙或添加非特异性钙粘蛋白拮抗剂肽可显著减少聚集形成并增加细胞凋亡导致的细胞死亡（分别为34 +/- 6%对75 +/- 1%和19 +/- 1%对40 +/- 5%）
Objective-Vascular smooth muscle cell (VSMC) apoptosis is thought to contribute to atherosclerotic plaque instability. Cadherin mediates calcium-dependent homophilic cell-cell contact. We studied the role of N-cadherin in VSMC apoptosis.Methods and Results-Human saphenous vein VSMCs were grown in agarose-coated wells to allow cadherin-mediated aggregate formation. Cell death and apoptosis were determined after disruption of cadherins using several approaches (n >= 3 per approach). Calcium removal from culture medium or addition of nonspecific cadherin antagonist peptides significantly decreased aggregate formation and increased cell death by apoptosis (34 +/- 6% versus 75 +/- 1% and 19 +/- 1% versus 40 +/- 5%, respectively; P