Detection and multigenic characterization of a novel gammaherpesvirus in goats
Detection and multigenic characterization of a novel gammaherpesvirus in goats
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DOI：
10.1016/s0168-1702(00)00252-5
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发表时间：
2001-05-01
期刊：
影响因子：
5
通讯作者：
Ehlers, B
中科院分区：
文献类型：
--
作者：
Chmielewicz, B;Goltz, M;Ehlers, B
用退化引物和脱氧肌苷取代引物对山羊外周血白细胞进行PCR检测，检测编码糖蛋白B （gB）、DNA聚合酶（DPOL）和终止酶（TERM）的疱疹病毒基因，获得了山羊γ -疱疹病毒存在的证据。然后用特定引物确定了从gB到DPOL基因的连续3.6 kbp序列。所有序列（gB、DPOL和TERM）均与伽玛疱疹病毒科对应基因有密切关系。氨基酸序列比对显示，该病毒与羊疱疹病毒2型的同源性特别高（> 83%），其次是羊疱疹病毒1型（> 76%）和牛嗜淋巴疱疹病毒（> 61%）。系统发育分析证实了这些关系。这些序列来源于假定的新型山羊疱疹病毒，暂定名为山羊疱疹病毒2号。这种病毒是在山羊身上发现的第一个伽玛疱疹病毒。(C) 2001 Elsevier Science B.V.版权所有
Evidence for the existence of a caprine gammaherpesvirus was obtained by analysis of peripheral blood leucocytes of goats with PCR assays that target the herpesvirus genes encoding the glycoprotein B (gB), the DNA polymerase (DPOL) and the terminase (TERM) with degenerate and deoxyinosine-substituted primers. A contiguous 3.6 kbp sequence extending from the gB to the DPOL gene was then determined with specific primers. All sequences (gB, DPOL and TERM) showed a close relationship with the corresponding genes of the Gammaherpesvirinae. Alignment of amino acid sequences revealed a particularly high percentage of identity with the ovine herpesvirus type 2 ( > 83%), followed by the alcelaphine herpesvirus 1 ( > 76%) and the bovine lymphotropic herpesvirus (> 61%). Phylogenetic analyses confirmed these relationships. The putative novel goat herpesvirus from which these sequences originate was tentatively designated caprine herpesvirus 2. This virus is the first gammaherpesvirus recognized in goats. (C) 2001 Elsevier Science B.V. All rights reserved.