Necessity of a functional octadecanoic pathway for indole alkaloid synthesis by Catharanthus roseus cell suspensions cultured in an auxin-starved medium
Necessity of a functional octadecanoic pathway for indole alkaloid synthesis by Catharanthus roseus cell suspensions cultured in an auxin-starved medium
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DOI：
10.1093/oxfordjournals.pcp.a029360
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发表时间：
1998-02-01
影响因子：
4.9
通讯作者：
Doireau, P
中科院分区：
文献类型：
--
作者：
Gantet, P;Imbault, N;Doireau, P
研究了十八烷酸途径的衍生物茉莉酸甲酯（mJA）、茉莉酸和愈伤酸对长春花细胞悬浮培养产生生物碱的影响。(G)在生长素存在下培养的细胞（m-细胞）不积累生物碱，向m-细胞中添加外源mJA恢复了产生生物碱的能力，在2，4-D饥饿培养基中培养的细胞（p-细胞）中，外源mJA大大增加了生物碱的产生，茉莉酸获得了类似的数据，相反，创伤酸对生物碱的产生没有影响，细胞悬浮培养物对外源mJA的敏感性仅限于继代培养的前4天，对应于活跃生长期，而生物碱积累仅发生在继代培养的稳定期当用十八烷酸途径抑制剂处理β-细胞时，产生生物碱的能力强烈降低，这些数据表明，响应于生长素耗尽，内源性mJA可以产生并通过连接生理事件起作用，从而导致生物碱生物合成激活。
The effect of methyl jasmonate (mJA), jasmonic acid and traumatic acid, derivatives of the octadecanoic pathway, on the production of alkaloids by cell suspension cultures of Catharanthus roseus L. (G) Don was investigated, Cells cultured in the presence of auxin (m-cells) did not accumulate alkaloids, The addition of exogenous mJA to m-cells restored the ability to produce alkaloids, In cells cultured in a 2,4-D-starved medium (p-cells), exogenous mJA greatly increased alkaloid production, Similar data were obtained for jasmonic acid, In contrast, traumatic acid had no effect on alkaloid production, The sensitivity of cell suspension cultures to exogenous mJA was restricted to the first four days of subculture corresponding to the active growth phase, whereas the alkaloid accumulation occurred only during the stationary phase of the subculture (days 6 to 10), When p-cells were treated with octadecanoic pathway inhibitors, the ability to produce alkaloids was strongly reduced, The addition of exogenous mJA always restored the ability to produce alkaloids, These data suggest that in response to auxin depletion, endogenous mJA could be produced and act by linking physiological events thus leading to alkaloid biosynthesis activation.