CLONING OF AN INTRINSIC HUMAN TFIID SUBUNIT THAT INTERACTS WITH MULTIPLE TRANSCRIPTIONAL ACTIVATORS
CLONING OF AN INTRINSIC HUMAN TFIID SUBUNIT THAT INTERACTS WITH MULTIPLE TRANSCRIPTIONAL ACTIVATORS
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DOI：
10.1126/science.7824954
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发表时间：
1995-01-27
期刊：
影响因子：
56.9
通讯作者：
ROEDER, RG
中科院分区：
文献类型：
--
作者：
CHIANG, CM;ROEDER, RG
TFIID是由TATA结合蛋白（TBP）和多种TBP相关因子（TAF）组成的多亚基蛋白复合物。TFIID中的TAF对于激活子依赖性转录是必需的，现在描述了编码人TFIID TAF，TAF（II）55的互补DNA的克隆，其在果蝇TFIID中没有已知的同源物。TAF（II）55通过其中心区域与人TFIID的最大亚基（TAF（II）230）相互作用，并通过不同的氨基末端结构域与多种激活剂（包括Sp1、YY1、USF、CTF、腺病毒E1 A和人免疫缺陷病毒1型达特蛋白）相互作用。Sp1的TAF（II）55相互作用区域定位于其DNA结合结构域，这与先前显示与果蝇TAF（II）110相互作用的富含谷氨酰胺的激活结构域不同。因此，这种人TFIID TAF可能是通过不同机制介导对多种激活剂的应答的共激活剂。
TFIID is a multisubunit protein complex comprised of the TATA-binding protein (TBP) and multiple TBP-associated factors (TAFs). The TAFs in TFIID are essential for activator-dependent transcription, The cloning of a complementary DNA encoding a human TFIID TAF, TAF(II)55, that has no known homolog in Drosophila TFIID is now described. TAF(II)55 is shown to interact with the largest subunit(TAF(II)230) of human TFIID through its central region and with multiple activators-including Sp1, YY1, USF, CTF, adenoviral E1A, and human immunodeficiency virus-type 1 Tat proteins-through a distinct amino-terminal domain. The TAF(II)55-interacting region of Sp1 was localized to its DNA-binding domain, which is distinct from the glutamine-rich activation domains previously shown to interact with Drosophila TAF(II)110. Thus, this human TFIID TAF may be a co-activator that mediates a response to multiple activators through a distinct mechanism.