Obtaining transgenic plants using the bio-active beads method
Obtaining transgenic plants using the bio-active beads method
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DOI：
10.1007/s10265-003-0141-3
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发表时间：
2004-02
影响因子：
2.8
通讯作者：
Haibo Liu;A. Kawabe;S. Matsunaga;T. Murakawa;A. Mizukami;Masanobu Yanagisawa;E. Nagamori;S. Harashima;A. Kobayashi;K. Fukui
中科院分区：
文献类型：
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作者：
Haibo Liu;A. Kawabe;S. Matsunaga;T. Murakawa;A. Mizukami;Masanobu Yanagisawa;E. Nagamori;S. Harashima;A. Kobayashi;K. Fukui
目前有几种转化方法可用于将外源DNA递送到动物和植物细胞中。在这项研究中，开发了一种新的和有效的转化系统的DNA交付/表达的能力，运输高分子量的DNA。该系统克服了传统的转化方法如农杆菌介导法、基因枪法和电转化法的缺点。本研究开发的方法使用海藻酸钙微珠结合聚乙二醇处理来包裹DNA分子。此外，它简单、成本低，需要的设备有限。利用这种方法，我们已经成功地转化烟草植株，通过卡那霉素抗性筛选。转化子中的转化基因通过PCR和Southern杂交证实。
Several methods of transformation are currently available for delivering exogenous DNA into animal and plant cells. In this study, a novel and efficient transformation system for DNA delivery/expression with a capacity to transport DNA of high molecular weight was developed. This system can overcome the shortcomings of traditional transformation methods such asAgrobacterium-mediated transformation, particle bombardment, and the electroporation method. The method developed in this study uses calcium alginate micro beads to immobilize DNA molecules in combination with polyethylene glycol treatment. In addition, it is simple and low-cost, and requires limited equipment. Using this method, we have successfully transformed tobacco plants, screening by kanamycin resistance. The transformed genes in the transformants were confirmed by PCR and Southern hybridization.