LATRUNCULINS - NOVEL MARINE TOXINS THAT DISRUPT MICROFILAMENT ORGANIZATION IN CULTURED-CELLS
LATRUNCULINS - NOVEL MARINE TOXINS THAT DISRUPT MICROFILAMENT ORGANIZATION IN CULTURED-CELLS
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DOI：
10.1126/science.6681676
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发表时间：
1983-01-01
期刊：
影响因子：
56.9
通讯作者：
GROWEISS, A
中科院分区：
文献类型：
--
作者：
SPECTOR, I;SHOCHET, NR;GROWEISS, A
两种毒素，latrunculins A和B，其中包含一类新的16-和14-元海洋大环内酯连接到罕见的2-噻唑烷酮部分，最近从红海海绵Latrunculia magnifica纯化。这些毒素对培养的小鼠神经母细胞瘤和成纤维细胞的影响进行了评价。在这两种类型的细胞，submicromolar毒素浓度迅速诱导细胞形态的显着变化，是可逆的毒素去除后。用细胞骨架蛋白特异性抗体进行的免疫荧光研究表明，毒素对微管系统的组织无明显影响，但会引起微丝组织的重大改变。
Two toxins, latrunculins A and B, which contain a new class of 16- and 14-membered marine macrolides attached to the rare 2-thiazolidinone moiety, were purified recently from the Red Sea sponge Latrunculia magnifica. The effects of these toxins on cultured mouse neuroblastoma and fibroblast cells were evaluated. In both types of cells, submicromolar toxin concentrations rapidly induced striking changes in cell morphology that were reversible upon removal of the toxin. Immunofluorescence studies with antibodies specific for cytoskeletal proteins revealed that the toxins caused major alterations in the organization of microfilaments without obvious effects on the organization of the microtubular system.