Protective effects of astaxanthin on lipopolysaccharide-induced inflammation in bovine endometrial epithelial cells†
Protective effects of astaxanthin on lipopolysaccharide-induced inflammation in bovine endometrial epithelial cells†
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DOI：
10.1093/biolre/ioz187
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发表时间：
2019-09
影响因子：
3.6
通讯作者：
F. Wan;Chen Zhang;Q. Jin;Chen Wei;Hongbo Zhao;Xianglun Zhang;W. You;Xiaomu Liu;Guifen Liu-Guif
中科院分区：
文献类型：
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作者：
F. Wan;Chen Zhang;Q. Jin;Chen Wei;Hongbo Zhao;Xianglun Zhang;W. You;Xiaomu Liu;Guifen Liu-Guif
虾青素（Astaxanthin， AST）是一种天然抗氧化类胡萝卜素，具有抗炎作用。然而，据我们所知，没有研究专门解决了AST对牛子宫内膜炎的潜在保护作用。本研究的目的是研究AST治疗是否可以保护子宫内膜上皮细胞免受脂多糖（LPS）诱导的炎症损伤。AST处理牛子宫内膜（BEND）上皮细胞系可降低lps诱导的白细胞介素-6和肿瘤坏死因子- α的产生，提高细胞超氧化物歧化酶和过氧化氢酶的活性，降低凋亡细胞比例，促进胰岛素样生长因子和上皮生长因子的产生。AST的作用是通过下调b细胞淋巴瘤2 （Bcl-2）相关的X、凋亡调节因子（Bax）和cleaved caspase-3以及上调Bcl-2介导的。此外，AST显著增加了紧密连接蛋白（TJP） claudin、cadherin-1和TJP1的表达，这些蛋白在维持宿主子宫内膜防御病原体感染的防御屏障中发挥重要作用。综上所述，这些结果表明，除了激活子宫内膜防御屏障外，AST治疗还可以防止氧化应激，防止细胞凋亡，促进BEND细胞活力，增加生长因子的产生。因此，AST是一种很有前景的治疗子宫内膜炎的药物。这一发现在当今时代是至关重要的，因为抗生素的过度使用导致了抗生素耐药细菌的发展。虾青素可以抵消lps诱导的牛子宫内膜上皮细胞的炎症、氧化应激、凋亡和紧密连接解体，是一种潜在的治疗牛子宫内膜炎的药物。
Abstract Astaxanthin (AST), a natural antioxidant carotenoid, has been shown to exert anti-inflammatory effects. However, to our knowledge, no study has specifically addressed the potential protective effects of AST against bovine endometritis. The purpose of this study was to examine whether treatment with AST could protect endometrial epithelial cells against lipopolysaccharide (LPS)-induced inflammatory injury. Treatment of bovine endometrial (BEND) epithelial cell line with AST reduced LPS-induced production of interleukin-6 and tumor necrosis factor-alpha, increased the cellular activity of superoxide dismutase and catalase, decreased the proportion of apoptotic cells, and promoted the production of insulin-like growth factor and epithelial growth factor. The effects of AST were mediated through the downregulation of B-cell lymphoma 2 (Bcl-2) associated X, apoptosis regulator (Bax), and cleaved caspase-3 and through the upregulation of Bcl-2. Moreover, AST significantly increased the expression of the tight junction proteins (TJP) claudin, cadherin-1, and TJP1, which play an essential role in the maintenance of host endometrial defense barrier against pathogen infection. Collectively, these results demonstrated that treatment with AST protected against oxidative stress, prevented cell apoptosis, promoted BEND cells viability, and increased the production of growth factors, in addition to activating the endometrial defense barrier. Therefore, AST is a promising therapeutic agent for the prevention and treatment of endometritis. This finding is of utmost importance in the present times when the excessive use of antibiotics has resulted in the development of antibiotic-resistant bacteria. Summary sentence Astaxanthin counteracts LPS-induced inflammation, oxidative stress, apoptosis, and tight junction disassembly in bovine endometrial epithelial cells, and is a potential therapeutic agent against bovine endometritis.