Huaier Extract Induces Apoptosis in Hepatoblastoma Cells Via the MEK/ERK Signaling Pathway
Huaier Extract Induces Apoptosis in Hepatoblastoma Cells Via the MEK/ERK Signaling Pathway
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DOI：
10.21873/invivo.12051
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发表时间：
2020-09-01
期刊：
影响因子：
2.3
通讯作者：
Toyoda, Hidemi
中科院分区：
文献类型：
--
作者：
Xu, Dong-Qing;Yuan, Xiao-Jun;Toyoda, Hidemi
背景/目的：怀尔提取物对多种恶性肿瘤具有抗肿瘤活性，其主要活性成分为蛋白多糖。在本研究中，我们旨在阐明怀尔提取物对肝母细胞瘤细胞的作用。材料与方法：观察槐儿提取物对人肝母细胞瘤细胞株HepG2和HuH-6增殖的影响。结果：怀尔提取物对肝母细胞瘤细胞活力有明显的剂量依赖性降低。槐儿提取物诱导肝母细胞瘤细胞S期阻滞，上调细胞周期相关蛋白cyclin D1和cyclin D3的表达。它还能诱导肝母细胞瘤细胞凋亡。显著抑制p-ERK和p-MEK活性。结论：槐儿提取物通过MEK-ERK通路抑制肝母细胞瘤细胞增殖，诱导细胞凋亡和细胞周期阻滞。怀尔提取物可作为治疗肝母细胞瘤的补充剂。
Background/Aim: Huaier extract, whose main active constituent is the proteoglycan, has anti-tumor activity in several types of malignancies. In this study, we aimed to elucidate the effect of Huaier extract in hepatoblastoma cells. Materials and Methods: The effect of Huaier extract on the proliferation of human hepatoblastoma cell lines HepG2 and HuH-6, was examined. Results: Incubation with Huaier extract resulted in a marked, dose-dependent decrease in hepatoblastoma cell viability. Huaier extract induced S phase arrest in hepatoblastoma cells and upregulated the expression of the cell cycle related proteins cyclin D1 and cyclin D3. It also induced apoptosis in hepatoblastoma cells. Additionally, it significantly suppressed the activity of p-ERK and p-MEK. Conclusion: Huaier extract inhibits proliferation, and induces cell apoptosis and cell cycle arrest via the MEK-ERK pathway in hepatoblastoma cells. Huaier extract may act as a complementary agent for treating hepatoblastoma.