MITOTIC PHOSPHORYLATION OF THE OCT-1 HOMEODOMAIN AND REGULATION OF OCT-1 DNA-BINDING ACTIVITY
MITOTIC PHOSPHORYLATION OF THE OCT-1 HOMEODOMAIN AND REGULATION OF OCT-1 DNA-BINDING ACTIVITY
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DOI：
10.1126/science.1684878
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发表时间：
1991-12-20
期刊：
影响因子：
56.9
通讯作者：
HEINTZ, N
中科院分区：
文献类型：
--
作者：
SEGIL, N;ROBERTS, SB;HEINTZ, N
Oct-1 是一种转录因子，参与组蛋白 H2B 基因转录的细胞周期调控和其他细胞管家基因的转录。当细胞进入有丝分裂时，Oct-1 过度磷酸化，而当细胞退出有丝分裂时，有丝分裂特异性磷酸化会逆转。 Oct-1 同源结构域中的有丝分裂特异性磷酸化位点在体外被蛋白激酶 A 磷酸化。该位点的磷酸化与体内和体外的 Oct-1 DNA 结合活性的抑制相关。有丝分裂期间 Oct-1 DNA 结合的抑制表明有丝分裂期间可能发生转录全面抑制的机制。
Oct-1 is a transcription factor involved in the cell cycle regulation of histone H2B gene transcription and in the transcription of other cellular housekeeping genes. Oct-1 is hyperphosphorylated as cells enter mitosis, and mitosis-specific phosphorylation is reversed as cells exit mitosis. A mitosis-specific phosphorylation site in the homeodomain of Oct-1 was phosphorylated in vitro by protein kinase A. Phosphorylation of this site correlated with inhibition of Oct-1 DNA binding activity in vivo and in vitro. The inhibition of Oct-1 DNA binding during mitosis suggests a mechanism by which the general inhibition of transcription during mitosis might occur.