Transitions in distinct histone H3 methylation patterns at the heterochromatin domain boundaries
Transitions in distinct histone H3 methylation patterns at the heterochromatin domain boundaries
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DOI：
10.1126/science.1064150
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发表时间：
2001-08-10
期刊：
影响因子：
56.9
通讯作者：
Grewal, SIS
中科院分区：
文献类型：
--
作者：
Noma, K;Allis, CD;Grewal, SIS
真核生物基因组被组织成离散的结构和功能染色质结构域。在这里，我们显示了不同的位点特异性组蛋白H3甲基化模式定义了分裂酵母交配型基因座47千碱基区域内的等染色质和异染色质染色体区域。H3赖氨酸9甲基化(H3Lys(9))及其相互作用的Swi6蛋白严格定位于20kb的沉默异色区间。相反，H3赖氨酸4甲基化(H3Lys(4))是周围常染色质区域所特有的。沉默区间两侧的两个反向重复序列作为边界元素，标记异染色质和常染色质之间的边界。这些边界元件的缺失导致H3Lys(9)甲基化和Swi6扩展到邻近序列。此外，H3Lys(9)甲基化和相应的异染色质相关复合体阻止了沉默区域中的H3Lys(4)甲基化。
Eukaryotic genomes are organized into discrete structural and functional chromatin domains. Here, we show that distinct site-specific histone H3 methylation patterns define euchromatic and heterochromatic chromosomal domains within a 47-kilobase region of the mating-type locus in fission yeast. H3 methylated at lysine 9 (H3 Lys(9)), and its interacting Swi6 protein, are strictly localized to a 20-kilobase silent heterochromatic interval. In contrast, H3 methylated at lysine 4 (H3 LyS(4)) is specific to the surrounding euchromatic regions. Two inverted repeats flanking the silent interval serve as boundary elements to mark the borders between heterochromatin and euchromatin. Deletions of these boundary elements lead to spreading of H3 Lys(9) methylation and Swi6 into neighboring sequences. Furthermore, the H3 Lys(9) methylation and corresponding heterochromatin-associated complexes prevent H3 LyS(4) methylation in the silent domain.