NUCLEOTIDE-SEQUENCE OF A SPECTINOMYCIN ADENYLTRANSFERASE AAD(9) DETERMINANT FROM STAPHYLOCOCCUS-AUREUS AND ITS RELATIONSHIP TO AAD(3'') (9)
NUCLEOTIDE-SEQUENCE OF A SPECTINOMYCIN ADENYLTRANSFERASE AAD(9) DETERMINANT FROM STAPHYLOCOCCUS-AUREUS AND ITS RELATIONSHIP TO AAD(3'') (9)
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DOI：
10.1007/bf00383309
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发表时间：
1985-01-01
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影响因子：
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通讯作者：
MURPHY, E
中科院分区：
文献类型：
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作者：
MURPHY, E
测定了金黄色葡萄球菌转座子Tn554的SPC决定簇的核苷酸序列。该基因编码壮观霉素腺基转移酶，AAD(9)，介导对壮观霉素的抗性，但不介导对链霉素的抗性。该序列预测了一个260个氨基酸的蛋白质，分子量为28,943。壮观霉素敏感突变体(SPC-1)含有一个G.fwdarw。在第165位用苏氨酸(ACA)取代丙氨酸(GCA)的转变。预测的氨基酸序列与广泛分布的革兰氏阴性链霉素/壮观霉素腺苷转移酶AAD(3‘)(9)的氨基酸序列有36%的同源性，由AADA决定簇指定。
The nucleotide sequence of the spc determinant of the S. aureus transposon, Tn554, was determined. This gene encodes a spectinomycin adenyltransferase, AAD(9), that mediates resistance to spectinomycin but not to streptomycin. The sequence predicts a 260 amino acid protein of MW 28,943. A spectinomycin-sensitive mutant (spc-1) contains a G .fwdarw. A transition resulting in substitution of threonine (ACA) for alanine (GCA) at residue 165. The predicted amino acid sequence is 36% homologous to that of a widely distributed, gram-negative streptomycin/spectinomycin adenyltransferase, AAD(3'''') (9), specified by the aadA determinant.