The Photodegradation of Lignin Methoxyl C Promotes Fungal Decomposition of Lignin Aromatic C Measured with (13)C-CPMAS NMR.
The Photodegradation of Lignin Methoxyl C Promotes Fungal Decomposition of Lignin Aromatic C Measured with (13)C-CPMAS NMR.
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13C-CPMAS NMR 测定木质素甲氧基 C 的光降解促进木质素芳香族 C 的真菌分解
DOI：
10.3390/jof8090900
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发表时间：
2022-08-24
期刊：
影响因子：
--
通讯作者：
Tian X
中科院分区：
文献类型：
--
作者：
Yao B;Zeng X;Pang L;Kong X;Tian K;Ji Y;Sun S;Tian X
太阳辐射一直被认为是干旱和半干旱生态系统中凋落物分解的驱动因素。凋落物有机碳(C)的光降解取决于化学成分和水的可用性。然而，有机碳中响应太阳辐射与水脉冲相互作用的化学变化仍然未知。为了解释凋落物在UV-B、UV-A和水脉冲介导的光合有效辐射(PAR)作用下的化学成分的变化，我们用固体13C交叉极化魔角旋转(CPMAS)核磁共振(核磁共振)技术测量了凋落物分解494天的凋落物的化学组成。非生物和生物因素通过三条途径调控凋落物的分解：第一，紫外光照射下木质素甲氧基C而不是芳香族C的光化学矿化；第二，PAR和UV辐射下纤维素O-烷基C的生物氧化和淋溶与水脉冲相互作用；第三，在辐射和水脉冲的相互作用下，UV辐射对木质素芳香C而不是纤维素O-烷基C的光毒害效应。能够解释质量损失变化的稳健分解指数是辐射下芳香C/O-烷基C的比率(AR/OA)，而辐射下的疏水与亲水C的比率(疏水性)、碳水化合物C/甲氧基C的比率(CC/MC)以及烷基C/O-烷基C的比率(A/OA)受水脉冲的影响。此外，光敏化效应和水分供应促进了与真菌分泌的木质素降解相关的过氧化物酶和酚氧化酶的潜在活性。我们的结果表明，木质素甲氧基C的直接光降解增加了微生物对木质素芳香族C的可及性。光氧化化合物可能是一个额外的碳库，通过降解木质素甲氧基和芳香族C来调节植物凋落物来源的土壤碳库的稳定性。
Solar radiation has been regarded as a driver of litter decomposition in arid and semiarid ecosystems. Photodegradation of litter organic carbon (C) depends on chemical composition and water availability. However, the chemical changes in organic C that respond to solar radiation interacting with water pulses remain unknown. To explain changes in the chemical components of litter organic C exposed to UV-B, UV-A, and photosynthetically active radiation (PAR) mediated by water pulses, we measured the chemistry of marcescent Lindera glauca leaf litter by solid-state 13C cross-polarization magic angle spinning (CPMAS) nuclear magnetic resonance (NMR) over 494 days of litter decomposition with a microcosm experiment. Abiotic and biotic factors regulated litter decomposition via three pathways: first, photochemical mineralization of lignin methoxyl C rather than aromatic C exposed to UV radiation; second, the biological oxidation and leaching of cellulose O-alkyl C exposed to PAR and UV radiation interacts with water pulses; and third, the photopriming effect of UV radiation on lignin aromatic C rather than cellulose O-alkyl C under the interaction between radiation and water pulses. The robust decomposition index that explained the changes in the mass loss was the ratio of aromatic C to O-alkyl C (AR/OA) under radiation, but the ratio of hydrophobic to hydrophilic C (hydrophobicity), the carbohydrate C to methoxyl C ratio (CC/MC), and the alkyl C to O-alkyl C ratio (A/OA) under radiation were mediated by water pulses. Moreover, the photopriming effect and water availability promoted the potential activities of peroxidase and phenol oxidase associated with lignin degradation secreted by fungi. Our results suggest that direct photodegradation of lignin methoxyl C increases microbial accessibility to lignin aromatic C. Photo-oxidized compounds might be an additional C pool to regulate the stability of the soil C pool derived from plant litter by degrading lignin methoxyl and aromatic C.
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影响因子：
30.7
作者：
Bradford, Mark A.;Warren, Robert J., II;King, Joshua R.
通讯作者：
King, Joshua R.
影响因子：
3.7
作者：
Brandt, L. A.;King, J. Y.;Sinsabaugh, R. L.
通讯作者：
Sinsabaugh, R. L.
紫外线辐照度的正面和负面影响解释了半干旱沙丘生态系统中凋落物位置和紫外线照射对凋落物分解和营养动态的相互作用
影响因子：
9.7
作者：
Erdenebileg, Enkhmaa;Ye, Xuehua;Cornelissen, Johannes H. C.
通讯作者：
Cornelissen, Johannes H. C.
影响因子：
9.2
作者：
Berenstecher, Paula;Vivanco, Lucia;Austin, Amy T.
通讯作者：
Austin, Amy T.
影响因子：
9.7
作者：
Bonanomi, Giuliano;Incerti, Guido;Mazzoleni, Stefano
通讯作者：
Mazzoleni, Stefano