Gene expression response to EWS-FLI1 in mouse embryonic cartilage.
Gene expression response to EWS-FLI1 in mouse embryonic cartilage.
复制标题
基因表达对小鼠胚胎软骨中EWS-FLI1的反应。
DOI：
10.1016/j.gdata.2014.09.003
复制
发表时间：
2014-12
期刊：
影响因子：
--
通讯作者：
Nakamura T
中科院分区：
文献类型：
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作者：
Tanaka M;Aisaki K;Kitajima S;Igarashi K;Kanno J;Nakamura T
尤文氏肉瘤是一种罕见的骨肿瘤，影响儿童和青少年。最近，我们成功地诱导尤文肉瘤样小圆细胞肿瘤在小鼠胚胎骨软骨祖细胞中表达EWS-ETS融合基因。尤文氏肉瘤前体细胞在长骨的胚胎表浅区（eSZ）细胞中富集。为了深入了解尤文肉瘤的发展机制，使用DNA微阵列比较了EWS-FLI 1敏感的eSZ细胞和EWS-FLI 1抗性的胚胎生长板（eGP）细胞之间的基因表达谱。在基因转导后0、8或48小时，在有或没有EWS-FLI 1表达的情况下评估eSZ和eGP细胞（总共，30个样品）的基因表达。我们的数据提供了有用的信息，融合癌基因在人类肉瘤的基因表达反应。
Ewing's sarcoma is a rare bone tumor that affects children and adolescents. We have recently succeeded to induce Ewing's sarcoma-like small round cell tumor in mice by expression of EWS–ETS fusion genes in murine embryonic osteochondrogenic progenitors. The Ewing's sarcoma precursors are enriched in embryonic superficial zone (eSZ) cells of long bone. To get insights into the mechanisms of Ewing's sarcoma development, gene expression profiles between EWS–FLI1-sensitive eSZ cells and EWS–FLI1-resistant embryonic growth plate (eGP) cells were compared using DNA microarrays. Gene expression of eSZ and eGP cells (total, 30 samples) was evaluated with or without EWS–FLI1 expression 0, 8 or 48 h after gene transduction. Our data provide useful information for gene expression responses to fusion oncogenes in human sarcoma.