Molecular cloning and characterization of a unique type of human papillomavirus from an immune deficient patient.
Molecular cloning and characterization of a unique type of human papillomavirus from an immune deficient patient.
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来自免疫缺陷患者的独特类型人乳头瘤病毒的分子克隆和表征。
DOI：
10.1111/1523-1747.ep12260698
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发表时间：
1984
期刊：
影响因子：
--
通讯作者：
Faras,AJ
中科院分区：
文献类型：
--
作者：
Ostrow,RS;Zachow,KR;Thompson,O;Faras,AJ
对一位表现出不寻常的免疫缺陷综合征的患者的几个乳头瘤进行了分析，以确定是否存在特定的人乳头瘤病毒（HPV）类型。初步分析表明，这些组织中存在的HPV DNA物种与先前表征的HPV类型完全不同。为了更严格地分析该患者的HPV，我们通过分子克隆将其分离到噬菌体lambda载体中，并构建了详细的限制性内切酶图谱。使用SI核酸酶分析的比较杂交研究显示，该病毒DNA与该实验室分子克隆的HPV 1、2、3、4、5、6型或HPV-11型具有6%或更少的核苷酸序列同源性。此外，在严格的杂交条件下，Southern blot分析显示，HPV 1、2、4、5、7、8、10、11型，从疣状表皮发育不良（EV）患者中分离的HPV-EV，或先前描述的与HPV-3相关的2种HPV （HPV- p和HPV- pw）几乎没有杂交。然而，与HPV-6的同源性非常弱，与HPV-3的同源性非常弱。未观察到与最近表征的hpv 9或-12至-24的过滤杂交。这些数据累积表明，该免疫抑制患者的HPV物种代表了一种新的，迄今尚未确定的HPV类型。
Several papillomas from a single patient who exhibited an unusual immune deficiency syndrome were analyzed for the presence of specific human papillomavirus (HPV) types. Preliminary analysis indicated that the HPV DNA species present in each of these tissues was quite unlike any of the previously characterized HPV types. In order to more rigorously analyze the HPV from this patient we have isolated the HPV DNA by molecularly cloning it into a bacteriophage lambda vector and have constructed a detailed restriction endonuclease map. Comparative hybridization studies using SI nuclease analyses showed 6% or less nucleotide sequence homology of this viral DNA with HPV types 1, 2, 3, 4, 5, 6, or an HPV-11, molecularly cloned in this laboratory. Moreover, Southern blot analyses under stringent hybridization conditions revealed little, if any, hybridization to HPV types 1, 2, 4, 5, 7, 8, 10, 11, HPV-EV isolated from a patient with epidermodysplasia verruciformis (EV), or 2 previously described HPVs (HPV-P and HPV-PW) related to HPV-3. There was, however, a very weak sequence homology detected with HPV-6 and an extremely weak homology to HPV-3. No filter hybridization was observed with the recently characterized HPVs 9 or -12 to -24. These data accumulatively indicate that the HPV species from this immunosuppressed patient represents a new, hitherto unidentified HPV type.