The TOPLESS corepressor regulates developmental switches in the bryophyte Physcomitrium patens that were critical for plant terrestrialisation
The TOPLESS corepressor regulates developmental switches in the bryophyte Physcomitrium patens that were critical for plant terrestrialisation
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DOI：
10.1111/tpj.16322
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发表时间：
2023-06-08
期刊：
影响因子：
7.2
通讯作者：
Davies，Brendan
中科院分区：
文献类型：
--
作者：
Causier，Barry;McKay，Mary;Davies，Brendan
植物特异性转录辅抑制因子TOPLESS（TPL）家族是多种被子植物发育过程的组成部分。尽管如此，我们对TPL在其他植物中的功能知之甚少。为了解决这一差距，我们研究了TPL在bratitePhyscomitrium patens中的作用，该物种约在5亿年前从被子植物中分化出来。尽管PpTPL功能的完全丧失是致命的，但PpTPL活性降低的转基因株系揭示了PpTPL对于这种植物中的两个基本发育开关是必不可少的：从基本光合细丝（chloronemata）到专门的觅食细丝（caulonemata）的转变以及从二维（2D）到三维（3D）生长。使用转录组学方法，我们将PpTPL整合到控制3D生长的调控网络中，我们认为PpTPL代表了另一类重要的调控因子，这些调控因子对于2D到3D的发育转换至关重要。转录组学还揭示了PpTPL在类黄酮调节中的一个先前未知的作用。有趣的是，3D生长和茎线的形成是促进植物在土地上殖民的关键创新，这是地球生命历史上的一个重大变革事件。我们的结论是，在陆地植物之前就存在的TPL被纳入了新的发展途径，使植物陆地化和陆地植物的进化成为可能。
The plant‐specific TOPLESS (TPL) family of transcriptional corepressors is integral to multiple angiosperm developmental processes. Despite this, we know little about TPL function in other plants. To address this gap, we investigated the roles TPL plays in the bryophytePhyscomitrium patens, which diverged from angiosperms approximately 0.5 billion years ago. Although complete loss of PpTPL function is lethal, transgenic lines with reduced PpTPL activity revealed that PpTPLs are essential for two fundamental developmental switches in this plant: the transitions from basal photosynthetic filaments (chloronemata) to specialised foraging filaments (caulonemata) and from two‐dimensional (2D) to three‐dimensional (3D) growth. Using a transcriptomics approach, we integrated PpTPL into the regulatory network governing 3D growth and we propose that PpTPLs represent another important class of regulators that are essential for the 2D‐to‐3D developmental switch. Transcriptomics also revealed a previously unknown role for PpTPL in the regulation of flavonoids. Intriguingly, 3D growth and the formation of caulonemata were crucial innovations that facilitated the colonisation of land by plants, a major transformative event in the history of life on Earth. We conclude that TPL, which existed before the land plants, was co‐opted into new developmental pathways, enabling phytoterrestrialisation and the evolution of land plants.