Global Proteomic Analysis of Saccharomyces cerevisiae Identifies Molecular Pathways of Histone Modifications
Global Proteomic Analysis of Saccharomyces cerevisiae Identifies Molecular Pathways of Histone Modifications
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DOI：
10.1007/978-1-59745-540-4_10
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发表时间：
2009-01-01
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影响因子：
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通讯作者：
Shilatifard, Ali
中科院分区：
文献类型：
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作者：
Jackson, Jessica;Shilatifard, Ali
真核细胞的很长的DNA在被包装进细胞核后必须保持功能。尽管我们对这一过程知之甚少，但很明显，染色质及其翻译后修饰在其中起着关键作用。酿酒酵母为破译染色质修饰和转录调控的分子机制提供了强大的遗传和生化模型系统。在本章中，我们描述了一种新的方法，即酿酒酵母的全局蛋白质组学分析（GPS），用于全局分析适当组蛋白修饰所需的分子机制。由于染色质生物学中涉及的许多分子机制从酵母到人类都是高度保守的，因此GPS已被证明是鉴定染色质修饰分子途径的杰出方法。
The very long DNA of the eukaryotic cells must remain functional when packaged into the cell nucleus. Although we know very little about this process, it is clear at this time that chromatin and its post-translational modifications play a pivotal role. Yeast Saccharomyces cerevisiae provides a powerful genetic and biochemical model system for deciphering the molecular machinery involved in chromatin modification and transcriptional regulation. In this chapter, we describe a novel method, the Global Proteomic analysis in S. cerevisiae (GPS), for the global analysis of the molecular machinery required for proper histone modifications. Since many of the molecular machineries involved in chromatin biology are highly conserved from yeast to humans, GPS has proven to be an outstanding method for the identification of the molecular pathways involved in chromatin modifications.