LIPID-PHOSPHOACETYLMURAMYL-PENTAPEPTIDE AND LIPID-PHOSPHODISACCHARIDE-PENTAPEPTIDE - PRESUMED MEMBRANE TRANSPORT INTERMEDIATES IN CELL WALL SYNTHESIS
LIPID-PHOSPHOACETYLMURAMYL-PENTAPEPTIDE AND LIPID-PHOSPHODISACCHARIDE-PENTAPEPTIDE - PRESUMED MEMBRANE TRANSPORT INTERMEDIATES IN CELL WALL SYNTHESIS
复制标题
DOI：
10.1073/pnas.53.4.881
复制
发表时间：
1965-01-01
影响因子：
11.1
通讯作者：
STROMINGER, JL
中科院分区：
文献类型：
--
作者：
ANDERSON, JS;MATSUHASHI, M;STROMINGER, JL
在使用尿苷二磷酸（UDP）-乙酰胞壁酰-五肽和UDP-乙酰-葡糖胺作为底物的反应中合成金黄色葡萄球菌或溶壁微球菌中的细胞壁糖肽。无机磷酸盐和尿苷磷酸盐（UMP）是由前者底物形成的其他产物，而UDP是由后者形成的。反应的机理是磷酸乙酰胞壁酰-五肽转移到脂质，形成乙酰葡糖胺-乙酰胞壁酰（-五肽）-P-脂质和二硫代氨基甲烷-五肽转移到受体（推测是不完整的糖肽），释放无机磷酸盐和推测的脂质。这可能是细胞运输机制的一个例子。糖肽合成的特异性抑制剂万古霉素和瑞斯托霉素不干扰脂质中间体的形成。它们的作用位点是利用脂-磷-磷二酰-五肽合成糖肽。
Cell wall glycopeptide in Staphylococcus aureus or Micrococcus lysodeikticus is synthesized in a reaction using uridine-diphosphate (UDP)-acetylmuramyl-pentapeptide and UDP-acetyl-glucosamine as substrates. Inorganic phosphate and uridine-monophos-phate (UMP) are the other products formed from the former substrate while UDP is formed from the latter. The mechanism of the reaction is transfer of phosphoacetylmuramyl-pentapeptide to lipid, formation of acetylglucosaminyl-acetylmuramyl(-penta-peptide)-P-lipid and transfer of the disaccharide-pentapeptide to an acceptor (presumably an incomplete glycopeptide) with release of inorganic phosphate and presumably of lipid. It is probably on example of a cellular transport mechanism. Vancomycin and ristocetin which are specific inhibitors of glycopeptide synthesis do not interfere with formation of the lipid intermediates. Their locus of action is in the utilization of lipid-phos-phodisaccharide-pentapeptide for glycopeptide synthesis.