DgMab-6: Antihuman DGKgamma Monoclonal Antibody for Immunocytochemistry.
DgMab-6: Antihuman DGKgamma Monoclonal Antibody for Immunocytochemistry.
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DgMab-6：用于免疫细胞化学的抗人 DGKgamma 单克隆抗体。
DOI：
10.1089/mab.2018.0026
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发表时间：
2018
影响因子：
--
通讯作者：
Goto K.
中科院分区：
文献类型：
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作者：
Nakano T;Ogasawara S;Tanaka T;Hozumi Y;Yamaki A;Sakane F;Shirai Y;Nakamura T;Yanaka M;Yamada S;Kaneko MK;Kato Y;Goto K.
甘油二酯激酶（DGK）磷酸化甘油二酯（DG）产生磷脂酸（PA）。由于DG和PA都是细胞内的第二信使，因此DGK在调节DG和PA介导的两条信号通路的平衡中起着关键作用。据报道，DGKγ是10种哺乳动物DGK同工酶之一，参与白血病细胞分化、肥大细胞功能和膜运输。利用标记的表达载体和大鼠组织上的免疫组织化学的转染研究显示DGKγ定位于细胞质、质膜和高尔基体。然而，关于DGKγ天然蛋白在人体组织和细胞中的详细定位的研究报道较少。本研究开发了一种新的抗DGK γ单克隆抗体DgMab-6，该抗体可用于人培养细胞的免疫细胞化学研究。
Diacylglycerol kinase (DGK) phosphorylates diacylglycerol (DG) to produce phosphatidic acid (PA). Since both DG and PA serve as lipidic second messengers, DGK plays a pivotal role in regulating the balance of two signaling pathways mediated by DG and PA in cellular functions. Reportedly, DGKγ, one of the 10 mammalian DGK isozymes, is involved in leukemic cell differentiation, mast cell function, and membrane traffic. Transfection studies using tagged expression vectors and immunohistochemistry on rat tissues revealed that DGKγ localizes to the cytoplasm, plasma membrane, and Golgi apparatus. However, a limited number of studies reported the detailed localization of native protein of DGKγ in human tissues and cells. In this study, we developed a novel anti-DGKγ monoclonal antibody, DgMab-6, which is very useful in immunocytochemistry of human cultured cells.