Effect of interfacial structure based on grafting density of poly(2-methoxyethyl acrylate) on blood compatibility
Effect of interfacial structure based on grafting density of poly(2-methoxyethyl acrylate) on blood compatibility
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基于聚丙烯酸2-甲氧基乙酯接枝密度的界面结构对血液相容性的影响
DOI：
10.1016/j.colsurfb.2020.111517
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发表时间：
2021
期刊：
影响因子：
--
通讯作者：
Masaru Tanaka
中科院分区：
文献类型：
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作者：
Tomoya Ueda;Daiki Murakami;Masaru Tanaka
聚（丙烯酸2-甲氧基乙酯）（PMEA）是一种优异的血液相容性聚合物，在与水或磷酸盐缓冲盐水（PBS）的界面处表现出纳米级的相分离结构，并且纤维蛋白原的吸附受到抑制，尤其是在富水区域。为了了解基于 PMEA 接枝密度的界面结构与血液相容性之间的相关性，通过将硫醇封端的 PMEA 固定在金基底上，制备了密度受控的接枝 PMEA (gPMEA) 表面。 gPMEA 上吸附的纤维蛋白原量和粘附血小板数量首先随着接枝密度 (σ) 的增加而减少，但在 σ 约为 0.11 链/nm2 时达到最小值后又增加。 gPMEA/PBS界面的界面结构随接枝密度的变化而变化，在σ=0.11时获得最大富水区面积。 σ = 0.11 时的水接触角小于其他接枝密度时的水接触角。这些结果表明，聚合物的水合对于抑制血小板粘附非常有效，并且富含水的区域在 gPMEA 表面上表现出优异的血液相容性。这项工作清楚地表明PMEA的密度影响界面结构并对材料的血液相容性起着重要作用。
An excellent blood-compatible polymer, poly(2-methoxyethyl acrylate) (PMEA), exhibits nanometer-scale phase-separated structures at the interface with water or phosphate-buffered saline (PBS), and fibrinogen adsorption is suppressed, especially on the water-rich region. To understand the correlation between the interfacial structure based on the grafting density of PMEA and blood compatibility, grafted PMEA (gPMEA) surfaces with controlled density were prepared by immobilizing thiol-terminated PMEA on a gold substrate. The amount of adsorbed fibrinogen and the number of adhered platelets on gPMEAs decreased first with the increasing grafting density (σ), but increased after showed minimum at σ of approximately 0.11 chains/nm2. The interfacial structures of the gPMEA/PBS interface changed with grafting density, and the maximum area of water-rich region was obtained at σ = 0.11. The water contact angle at σ = 0.11 is smaller than that at the other grafting density. These results revealed that hydration to the polymer is very effective to suppress the platelet adhesion and water-rich region shows excellent blood compatibility on gPMEA surfaces. This work clearly indicated that the density of PMEA affects the interfacial structure and plays an important role in the blood compatibility of the material.