Phylogeny, Pathogenicity, Transmission, and Host Immune Responses of Four H5N6 Avian Influenza Viruses in Chickens and Mice
Phylogeny, Pathogenicity, Transmission, and Host Immune Responses of Four H5N6 Avian Influenza Viruses in Chickens and Mice
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DOI：
10.3390/v11111048
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发表时间：
2019-11
期刊：
影响因子：
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通讯作者：
Yafen Song;Weiqiang Li;Wenbo Wu;Zhiting Liu;Zhuoliang He;Zuxian Chen;Bingbing Zhao;Siyu Wu;Chenghuai Yang;Xiaoyun Qu;M. Liao;P. Jiao
中科院分区：
文献类型：
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作者：
Yafen Song;Weiqiang Li;Wenbo Wu;Zhiting Liu;Zhuoliang He;Zuxian Chen;Bingbing Zhao;Siyu Wu;Chenghuai Yang;Xiaoyun Qu;M. Liao;P. Jiao
H5 Nx病毒在世界范围内不断出现，给家禽业造成损失，并构成潜在的公共卫生风险。在这里，我们研究了2013年至2014年期间从水禽中分离的四种H5 N6禽流感病毒在鸡和小鼠中的繁殖、致病性、传播和免疫应答。他们的HA基因属于2008年在中国流传的进化枝2.3.4.4。它们的NA基因属于N6-like/Eurasian亚系。它们的内部基因来自不同的H5 N1病毒。结果表明，4株H5 N6病毒均为H5 N1和H6 N6病毒的复制株。它们在鸡中具有高传播能力，可引起致命感染。它们还在小鼠中引起轻度至严重的致病性，并可通过血脑屏障扩散到大脑。在感染过程中，病毒导致鸡和小鼠脑和肺中PRR和细胞因子的上调。结果提示，H5 N6病毒在鸡和小鼠体内的高病毒载量可导致疾病的严重程度;不同致病性的H5 N6病毒在鸡和小鼠体内可诱导不同水平的细胞因子。
H5Nx viruses have continuously emerged in the world, causing poultry industry losses and posing a potential public health risk. Here, we studied the phylogeny, pathogenicity, transmission, and immune response of four H5N6 avian influenza viruses in chickens and mice, which were isolated from waterfowl between 2013 and 2014. Their HA genes belong to Clade 2.3.4.4, circulated in China since 2008. Their NA genes fall into N6-like/Eurasian sublineage. Their internal genes originated from different H5N1 viruses. The results suggested that the four H5N6 viruses were reassortants of the H5N1 and H6N6 viruses. They cause lethal infection with high transmission capability in chickens. They also cause mild to severe pathogenicity in mice and can spread to the brain through the blood–brain barrier. During the infection, the viruses result in the up-regulation of PRRs and cytokine in brains and lungs of chickens and mice. Our results suggested that the high viral loads of several organs may result in disease severity in chickens and mice; there were varying levels of cytokines induced by the H5N6 viruses with different pathogenicity in chickens and mice.