A comparison of the immunomodulating properties of two forms of monophosphoryl lipid A analogues.
A comparison of the immunomodulating properties of two forms of monophosphoryl lipid A analogues.
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两种形式的单磷酰脂质 A 类似物的免疫调节特性的比较。
DOI：
10.1097/00002371-199112000-00002
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发表时间：
1991
期刊：
影响因子：
--
通讯作者：
Odean,M
中科院分区：
文献类型：
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作者：
Johnson,AG;Tomai,MA;Chen,YF;Odean,M
本研究比较了两种形式的单磷酰脂质A的免疫调节活性，它们是细菌脂多糖的类似物，毒性很小或没有毒性。测试的是命名为504的合成化合物和命名为MPL的从细菌细胞壁分离的纯化化合物。这两种临床佐剂候选物在小鼠中有效地在以下方面发挥强免疫调节活性：（a）增强年轻和老龄小鼠中的抗体产生;（B）在不同实验条件下抑制抗体形成;（c）激活巨噬细胞以分泌白细胞介素1、过氧化氢和超氧阴离子;和（d）刺激来自C3 H/HeN小鼠的脾细胞增殖。与天然脂多糖（LPS）相比，两者在LD 50测定中均表现出显著降低的毒性。MPL的LD 50为225倍，化合物504的LD 50为天然LPS在极敏感的、加载半乳糖胺的C57 BL/6鼠品系中的LD 50的40倍。
This investigation compared the immunomodulating activities of two forms of monophosphoryl lipid A, which are analogues of bacterial lipopolysaccharides with little or no toxicity. Tested were a synthetic compound designated 504 and a purified compound, isolated from bacterial cell walls designated MPL. Both of these clinical adjuvant candidates were effective in mice in exerting strong immunomodulating activity in the following areas:(a) enhancing antibody production in young and aging mice;(b) suppressing antibody formation under different experimental conditions;(c) activating macrophages to secrete interleukin 1, hydrogen peroxide, and superoxide anion; and (d) stimulating proliferation of spleen cells from C3H/HeN mice. Both exhibited considerably reduced toxicity in LD 50 assays when compared to native lipopolysaccharides (LPS). The LD 50 for MPL was 225 times and that of compound 504, 40 times that of native LPS in the exquisitely sensitive, galactosamine-loaded C57BL/6 murine strain.