Role of dimorphism in the development of Candida albicans biofilms
Role of dimorphism in the development of Candida albicans biofilms
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DOI：
10.1099/00222615-48-7-671
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发表时间：
1999-07-01
影响因子：
3
通讯作者：
Douglas, LJ
中科院分区：
文献类型：
--
作者：
Baillie, GS;Douglas, LJ
两种模型生物膜系统，包括在导管材料圆盘和圆柱形纤维滤膜上的生长，被用来研究白色念珠菌生物膜的结构。为了评价二形性在生物膜发育中的重要性，将两株野生型菌株产生的生物膜与两种形态突变体形成的生物膜进行了比较，两种形态突变体分别不能生长酵母和菌丝。扫描电子显微镜和光学显微镜下观察的生物膜薄片显示，野生型菌株在导管盘上形成的生物膜由两个不同的层组成：较薄的基础酵母层和较厚但更开放的菌丝层。菌丝(-)突变体只产生基底层，而酵母(-)突变体形成了更厚的菌丝生物膜，相当于野生型结构的外层。酵母突变株的生物膜比其他突变株更容易从导管表面脱落，这表明基础酵母层在将生物膜锚定到导管表面中起着重要作用。圆柱形纤维素过滤器上形成的生物膜在外观上有很大的不同。菌丝(-)突变体和两种野生型都只产生酵母型生物膜，而酵母(-)突变体在过滤器的顶部产生密集的菌丝垫。所有这些生物膜，无论形态如何，都对抗真菌剂两性霉素B具有耐药性。总体而言，这些结果表明白色念珠菌生物膜的结构取决于接触表面的性质，但一些表面产生的生物膜具有类似于细菌系统的分层结构。
Two model biofilm systems, involving growth on disks of catheter material or on cylindrical cellulose filters, were used to investigate the structure of Candida albicans biofilms. To assess the importance of dimorphism in biofilm development, biofilms produced by two wild-type strains were compared with those formed by two morphological mutants, incapable of yeast and hyphal growth, respectively. Scanning electron microscopy and thin sections of biofilms examined by light microscopy revealed that biofilms of the wild-type strains formed on catheter disks consisted of two distinct layers: a thin, basal yeast layer and a thicker, but more open, hyphal layer. The hypha(-) mutant produced only the basal layer, whereas the yeast(-) mutant formed a thicker, hyphal biofilm equivalent to the outer zone of the wild-type structures. Biofilms of the yeast- mutant were more easily detached from the catheter surface than the others, suggesting that the basal yeast layer has an important role in anchoring the biofilm to the surface. Biofilms formed on cylindrical cellulose filters were quite different in appearance. The hypha(-) mutant and both wild types produced exclusively yeast-form biofilms whereas the yeast(-) mutant generated a dense hyphal mat on the top of the filter. All these biofilms, irrespective of morphological form, were resistant to the antifungal agent, amphotericin B. Overall, these results indicate that the structure of a C. albicans biofilm depends on the nature of the contact surface, but that some surfaces produce biofilms with a layered architecture resembling to that described for bacterial systems.