Improvement of leukocyte functions in young prematurely aging mice after a 5-week ingestion of a diet supplemented with biscuits enriched in antioxidants
Improvement of leukocyte functions in young prematurely aging mice after a 5-week ingestion of a diet supplemented with biscuits enriched in antioxidants
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DOI：
10.1089/ars.2005.7.1203
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发表时间：
2005-09-01
影响因子：
6.6
通讯作者：
De la Fuente, M
中科院分区：
文献类型：
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作者：
Alvarado, C;Alvarez, P;De la Fuente, M
在我们以前的研究中，饮食中补充治疗性硫醇抗氧化剂改善了小鼠腹膜白细胞的功能，特别是在成年早衰小鼠（PAM）中。在目前的工作中，我们已经研究了在5周的饮食补充20%（重量/重量）的饼干富含抗氧化剂（维生素C，维生素E，β-胡萝卜素，锌，硒）的几个免疫功能的腹膜白细胞从年轻的PAM摄入的影响。结果表明，在巨噬细胞中，趋化性和吞噬作用以及细胞内自由基水平，这是抑制在PAM与对照非早衰小鼠（NPAM），补充后增加，特别是在PAM。淋巴细胞趋化性、对丝裂原伴刀豆球蛋白A的增殖反应和白细胞介素-2释放以及自然杀伤细胞活性也增加。然而，随着年龄的增长而增加的肿瘤坏死因子-α的释放在补充5周后减少。由于免疫系统的良好保存功能是健康和长寿的极好标志，因此摄入本饮食后白细胞功能的改善表明，这种抗氧化剂补充剂可能有助于老年人群的健康和功能性长寿。
In our previous studies, diet supplementation with therapeutic thiolic antioxidants improved the function of peritoneal leukocytes from mice, especially in adult prematurely aging mice (PAM). In the present work, we have studied the effects of ingestion during 5 weeks of a diet supplemented with 20% (wt/wt) of biscuits enriched with antioxidants (vitamin C, vitamin E, beta-carotene, zinc, and selenium) on several immune functions of peritoneal leukocytes from young PAM. The results show that, in macrophages, chemotaxis and phagocytosis as well as the intracellular free radical levels, which are depressed in PAM in comparison with the control non-prematurely aging mice (NPAM), increase after supplementation, especially in the PAM. An increase also occurs in lymphocyte chemotaxis, proliferative response to the mitogen concanavalin A, and interleukin-2 release, as well as in natural killer cell activity. However, the release of tumor necrosis factor-alpha, which increases with aging, decreases after 5 weeks of supplementation. As a well preserved function of the immune system is an excellent marker of health and longevity, the improvement of leukocyte functions after ingestion of the present diet suggests that this antioxidant supplementation may be useful for the preservation of health and functional longevity in aging populations.