Associations between sperm competition and natural variation in male reproductive genes on the third chromosome of Drosophila melanogaster
Associations between sperm competition and natural variation in male reproductive genes on the third chromosome of Drosophila melanogaster
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DOI：
10.1534/genetics.106.064915
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发表时间：
2007-06-01
期刊：
影响因子：
3.3
通讯作者：
Clark, Andrew G.
中科院分区：
文献类型：
--
作者：
Fiumera, Anthony C.;Dumont, Bethany L.;Clark, Andrew G.
我们应用关联分析来阐明自然种群的果蝇交配后性选择的表型变异的遗传基础。我们对96个第三染色体代换系进行了9种影响精子竞争能力的表型评分，并对它们进行了13个雄性生殖基因的72个多态性的基因分型。除雄性诱导的不应性（P = 0.053）外，所有表型在品系间均存在显著异质性（P < 0.01）。我们确定了24个关联（8个单标记关联，12个三标记单倍型关联，以及4例单标记相互作用揭示的上位性）。这些关联中只有不到9个可能是假阳性。几个协会是一致的，与以前的研究结果[Acp 70 A与男性的影响，女性的难治性再交配（难治性），酯酶-6，与男性的再交配概率（再交配）和女性后代生产的措施（生育力）]，但许多是新的协会与未表征的精液蛋白。四个基因显示出多效性效应的证据[CG 6168与精子竞争（P2 '）和难治性的测量，CG 14560与精子竞争（P1'）的防御性测量和雌性生殖力的测量，Acp 62 F与P2'和雌性生殖力的测量，以及酯酶-6与再交配和雌性生殖力的测量]。我们的研究结果提供的证据表明，多效性和上位性是男性生殖成功的遗传结构中的重要因素，并表明单倍型分析可以识别协会错过了单标记的方法。
We applied association analysis to elucidate the genetic basis for variation in phenotypes affecting postcopulatory sexual selection in a natural population of Drosophila melanogaster. We scored 96 third chromosome Substitution lines for nine phenotypes affecting sperm competitive ability and genotyped them at 72 polymorphisms in 13 male reproductive genes. Significant heterogeneity among lines (P < 0.01) was detected for all phenotypes except male-induced refractoriness (P = 0.053). We identified 24 associations (8 single-marker associations, 12 three-marker haplotype associations, and 4 cases of epistasis revealed by single-marker interactions). Fewer than 9 of these associations are likely to be false positives. Several associations were consistent with previous findings [Acp70A with the male's influence on the female's refractoriness to remating (refractory), Esterase-6,with a male's remating probability (remating) and a measure of female offspring production (fecundity)], but many are novel associations with uncharacterized seminal fluid proteins. Four genes showed evidence for pleiotropic effects [CG6168 with a measure of sperm competition (P2') and refractory, CG14560 with a defensive measure of sperm competition (P1') and a measure of female fecundity, Acp62F with P2' and a measure of female fecundity, and Esterase-6 with remating and a measure of female fecundity]. Our findings provide evidence that pleiotropy and epistasis are important factors in the genetic architecture of male reproductive success and show that haplotype analyses can identify associations missed in the single-marker approach.