Actin in the photoreceptor connecting cilium: immunocytochemical localization to the site of outer segment disk formation.
Actin in the photoreceptor connecting cilium: immunocytochemical localization to the site of outer segment disk formation.
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DOI：
10.1083/jcb.99.1.239
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发表时间：
1984-07
期刊：
影响因子：
--
通讯作者：
Papermaster DS
中科院分区：
文献类型：
--
作者：
Chaitin MH;Schneider BG;Hall MO;Papermaster DS
肌动蛋白已被定位在蛙pipiens视网膜固定和包埋在醛交联BSA。薄切片依次与（a）亲和纯化的兔诱导抗肌动蛋白抗体;（B）生物素-羊抗兔抗体;和（c）抗生物素蛋白-铁蛋白偶联物反应。正如预期的那样，antiactin标记密度高，在顶端色素上皮细胞的过程中，在光感受器的盏过程。肌动蛋白也定位在一个新的网站。连接视杆细胞和视锥细胞内外段的连接纤毛在其外段（OS）或远端被抗肌动蛋白大量标记。在纤毛的该区域，质膜外翻形成新的OS盘，并且在某些情况下这些基底盘被标记。在新的花盘下方，远端纤毛的唇状扩张的细胞质也被大量标记。连接纤毛的质膜和内部以及OS的其余部分未标记。这些研究结果表明，肌动蛋白可能参与载体运输的视蛋白和其他内在的膜蛋白，纳入新形成的OS磁盘。这些结果也暗示了肌动蛋白参与了OS盘形成的膜扩张。
Actin has been localized in Rana pipiens retinas that were fixed and embedded in aldehyde cross-linked BSA. Thin sections were reacted sequentially with (a) affinity-purified antiactin antibodies induced in rabbits; (b) biotinyl-sheep anti-rabbit antibodies; and (c) avidin- ferritin conjugates. As expected, antiactin labeling density was high in the apical pigment epithelial cell processes and in the calycal processes of photoreceptors. Actin was also localized in a new site. The connecting cilium that joins the inner and outer segments of both rods and cones was heavily labeled by antiactin at its outer segment (OS), or distal, end. In this region of the cilium, the plasma membrane evaginates to form new OS disks and these basal disks were labeled in some instances. Below the new disks in rods, the cytoplasm of liplike expansions of the distal cilium was also heavily labeled. The plasma membrane and interior of the connecting cilium and the remainder of the OS were unlabeled. These findings suggest that actin may participate in the vectorial transport of opsin and other intrinsic membrane proteins that are incorporated into newly forming OS disks. The results also implicate actin in the membrane expansion involved with OS disk formation.