Cloning of a cDNA for short/branched chain acyl-Coenzyme A dehydrogenase from rat and characterization of its tissue expression and substrate specificity.
Cloning of a cDNA for short/branched chain acyl-Coenzyme A dehydrogenase from rat and characterization of its tissue expression and substrate specificity.
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DOI：
10.1006/abbi.1996.0290
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发表时间：
1996-07
影响因子：
3.9
通讯作者：
J. Willard;C. Vicanek;K. Battaile;P. V. Van Veldhoven;A. Fauq;R. Rozen;J. Vockley
中科院分区：
文献类型：
--
作者：
J. Willard;C. Vicanek;K. Battaile;P. V. Van Veldhoven;A. Fauq;R. Rozen;J. Vockley
酰基辅酶A脱氢酶是催化酰基辅酶A酯的α，β-脱氢，将电子转移到电子转移黄素蛋白的相关酶家族。人短/支链酰基辅酶A脱氢酶的cDNA最近已被克隆，并且已经表明该酶代表先前报道的从大鼠肝脏纯化的2-甲基支链酰基辅酶A脱氢酶的人同源物。我们现在报告的大鼠短/支链酰基辅酶A脱氢酶的克隆和表达及其底物特异性的表征。大鼠酶对较长的碳侧链比其人类对应物更有活性，而人类酶可以利用具有较长初级碳链的底物。此外，短链/支链酰基-CoA脱氢酶可以利用丙戊酸-CoA作为底物。mRNA的北方印迹显示大鼠和人酶的普遍组织表达。对这些酶的进一步研究将有助于了解该基因家族的结构/功能关系。
The acyl-CoA dehydrogenases are a family of related enzymes which catalyze the alpha,beta-dehydrogenation of acyl-CoA esters, transferring electrons to electron-transferring flavoprotein. A cDNA for human short/branched chain acyl-CoA dehydrogenase has recently been cloned, and it has been suggested that this enzyme represents the human homolog for the previously reported 2-methyl branched chain acyl-CoA dehydrogenase purified from rat liver. We now report the cloning and expression of rat short/branched chain acyl-CoA dehydrogenase and characterization of its substrate specificity. The rat enzyme is more active toward longer carbon side chains than its human counterpart, while the human enzyme can utilize substrates with longer primary carbon chains. In addition, short/branched chain acyl-CoA dehydrogenase can utilize valproyl-CoA as a substrate. Northern blotting of mRNA shows ubiquitous tissue expression of both the rat and human enzyme. Further study of these enzymes will be helpful in understanding structure/function relationships in this gene family.