De Novo Assembly of the Transcriptome of Turritopsis, a Jellyfish That Repeatedly Rejuvenates
De Novo Assembly of the Transcriptome of Turritopsis, a Jellyfish That Repeatedly Rejuvenates
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DOI：
10.2108/zs150186
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发表时间：
2016-08-01
影响因子：
0.9
通讯作者：
Kubota, Shin
中科院分区：
文献类型：
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作者：
Hasegawa, Yoshinori;Watanabe, Takashi;Kubota, Shin
在大多数动物中，衰老是一个不可逆转的过程；然而，物种Turritopsis sp.已经观察到经历了多达14次的返老还童过程。在本研究中，我们利用多重RNA文库获得了Turritopsis sp.的四个发育阶段(ST)的转录组，包括(I)未成熟的水母、(II)饺子、(III)具短匍匐茎的饺子和(IV)新近恢复活力的息肉。总共有40.2亿对末端读数被从头组装起来，产生了90,327个重叠群。我们的分析表明，74%的组装重叠群得到了显著的BLAST点击，19%的重叠群成功地用基因本体(GO)术语进行了标注。BLAST搜索表明，32%的重叠群与普通九头蛇的序列最相似。每个样本的原始读数都与重叠群对应，以找到ST特异的基因。这代表了该物种的第一组全面的从头转录组数据，这可能为更好地理解多细胞动物的周期性恢复提供线索。
In most animals, aging is an irreversible process; however the species Turritopsis sp. has been observed to undergo a rejuvenation process as many as 14 times. In the present study, we used multiplexed RNA libraries to obtain the transcriptome from four developmental stages (St) of Turritopsis sp., including (I) immature medusa, (II) dumpling, (III) dumpling with a short stolon, and (IV) polyp, which had recently rejuvenated. A total of 4.02 billion paired-end reads were assembled de novo, yielding 90,327 contigs. Our analyses revealed that significant blast hits were recovered for 74% of the assembled contigs, and 19% were successfully annotated with gene ontology (GO) terms. A BLAST search demonstrated that 32% of the contigs were most similar to Hydra vulgaris-sequences. Raw reads from each sample were mapped against the contigs to find St-specific genes. This represents the first comprehensive set of de novo transcriptome data for this species, which may provide clues toward a better understanding of cyclical rejuvenation in multicellular animals.