Auxin-induced signaling protein nanoclustering contributes to cell polarity formation
Auxin-induced signaling protein nanoclustering contributes to cell polarity formation
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DOI：
10.1038/s41467-020-17602-w
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发表时间：
2020-08-06
影响因子：
16.6
通讯作者：
Yang, Zhenbiao
中科院分区：
文献类型：
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作者：
Pan, Xue;Fang, Linjing;Yang, Zhenbiao
细胞极性是真核生物和原核生物发育的基础，但其形成的机制还不是很清楚。在此，我们发现，植物激素生长素诱导的、依赖于类固醇的细胞表面跨膜受体激酶1(TMk1)在拟南芥子叶铺面细胞(PC)形态发生过程中对质膜(PM)极化域的形成至关重要。生长素诱导的TMk1纳米聚集稳定了Flotillin1相关的有序纳米结构域，这反过来又促进了ROP6 GTPase的纳米聚集，该酶作用于TMk1下游，调节皮质微管组织。反过来，皮质微管进一步稳定PM中含有TMk1和Flotillin1的纳团因此，我们提出了一种新的极性形成范式：扩散信号通过促进细胞表面受体介导的信号成分的纳米聚集和细胞骨架介导的正反馈将这些纳米结构域加强到极化结构域来触发细胞极化。蛋白质纳米聚集在细胞极化中的意义尚不清楚。在这里，潘等人。结果表明，生长素诱导的TMk1/固醇纳米簇以及基于微管的TMk1/固醇纳米簇的正反馈调节对于拟南芥细胞极性的形成至关重要。
Cell polarity is fundamental to the development of both eukaryotes and prokaryotes, yet the mechanisms behind its formation are not well understood. Here we found that, phytohormone auxin-induced, sterol-dependent nanoclustering of cell surface transmembrane receptor kinase 1 (TMK1) is critical for the formation of polarized domains at the plasma membrane (PM) during the morphogenesis of cotyledon pavement cells (PC) in Arabidopsis. Auxin-induced TMK1 nanoclustering stabilizes flotillin1-associated ordered nanodomains, which in turn promote the nanoclustering of ROP6 GTPase that acts downstream of TMK1 to regulate cortical microtubule organization. In turn, cortical microtubules further stabilize TMK1- and flotillin1-containing nanoclusters at the PM. Hence, we propose a new paradigm for polarity formation: A diffusive signal triggers cell polarization by promoting cell surface receptor-mediated nanoclustering of signaling components and cytoskeleton-mediated positive feedback that reinforces these nanodomains into polarized domains. The significance of protein nanoclustering in cell polarization is unclear. Here Pan et al. show that auxin-induced TMK1/sterol nanoclustering as well as microtubule-based positive feedback regulation of the TMK1/sterol nanoclusters is critical for cell polarity formation in Arabidopsis.