Plasticity in the proteome of Emiliania huxleyi CCMP 1516 to extremes of light is highly targeted
Plasticity in the proteome of Emiliania huxleyi CCMP 1516 to extremes of light is highly targeted
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DOI：
10.1111/nph.12352
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发表时间：
2013-10-01
期刊：
影响因子：
9.4
通讯作者：
Geider, Richard J.
中科院分区：
文献类型：
--
作者：
McKew, Boyd A.;Lefebvre, Stephane C.;Geider, Richard J.
在微藻生态生理学的理论研究中，经常应用最优性原理来预测资源在不同代谢途径中的分配变化，最优驯化可能涉及蛋白质组的变化，而蛋白质组通常占细胞氮(N)的50%。我们验证了微藻赫胥黎Emiliania huxleyi CCMP 1516对次优和超优光的适应涉及蛋白质组的巨大变化，因为细胞重新平衡了吸收光、固定二氧化碳、进行生物合成和抵抗光氧化应激的能力。在30 （LL）和1000 μ mol光子m(-2) s(-1) （HL）的连续培养条件下培养赫胥利Emiliania huxleyi，并采用LC-MS/MS shotgun蛋白质组学分析蛋白质组学的变化。变化最明显的是参与光合作用光反应的蛋白质；光系统I （PSI）和PSII蛋白的相对丰度在LL高70%，光收获型岩藻黄素-叶绿素蛋白（Lhcfs）的相对丰度在LL高500%，光保护型LI818蛋白的相对丰度在HL高300%。Lhcfs和LI818s丰度的显著变化，以及赫胥黎蛋白质组的有限可塑性，可能反映了该物种在自然界中遇到的随机光环境中提供能量以维持代谢能力的进化压力。
Optimality principles are often applied in theoretical studies of microalgal ecophysiology to predict changes in allocation of resources to different metabolic pathways, and optimal acclimation is likely to involve changes in the proteome, which typically accounts for >50% of cellular nitrogen (N).We tested the hypothesis that acclimation of the microalga Emiliania huxleyi CCMP 1516 to suboptimal vs supraoptimal light involves large changes in the proteome as cells rebalance the capacities to absorb light, fix CO2, perform biosynthesis and resist photooxidative stress.Emiliania huxleyi was grown in nutrient-replete continuous culture at 30 (LL) and 1000 mu mol photons m(-2) s(-1) (HL), and changes in the proteome were assessed by LC-MS/MS shotgun proteomics. Changes were most evident in proteins involved in the light reactions of photosynthesis; the relative abundance of photosystem I (PSI) and PSII proteins was 70% greater in LL, light-harvesting fucoxanthin-chlorophyll proteins (Lhcfs) were up to 500% greater in LL and photoprotective LI818 proteins were 300% greater in HL.The marked changes in the abundances of Lhcfs and LI818s, together with the limited plasticity in the bulk of the E. huxleyi proteome, probably reflect evolutionary pressures to provide energy to maintain metabolic capabilities in stochastic light environments encountered by this species in nature.