Immunolocalization and expression of lubricin in the bilaminar zone of the human temporomandibular joint disc
Immunolocalization and expression of lubricin in the bilaminar zone of the human temporomandibular joint disc
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DOI：
10.1016/j.acthis.2010.11.011
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发表时间：
2012-01-01
影响因子：
2.5
通讯作者：
Musumeci, Giuseppe
中科院分区：
文献类型：
--
作者：
Leonardi, Rosalia;Rusu, Mugurel Constantin;Musumeci, Giuseppe
本实验对关节边界润滑剂Lubricin在人颞下颌关节（TMJ）双板区（BZ）进行了组织化学研究。对17具尸体的33个颞下颌关节盘进行了润滑素免疫组化检测。将切片与稀释的兔多克隆抗润滑素抗体孵育，并根据润滑素免疫阳性细胞的百分比进行评分。分析了三种不同的颞下颌关节盘组织隔室，即：上板，下板和板间间隙的疏松结缔组织。Mann-Whitney U检验用于比较椎间盘标本区域之间的蛋白质表达（润滑素）。在每个椎间盘组织样本中的TMJ椎间盘细胞群中注意到染色，几乎每个细胞都被润滑素抗体免疫标记。免疫标记的椎间盘细胞数量在3个双板带区域几乎相同。细胞外基质染色阳性。本研究的结果表明，润滑素表达在颞下颌关节盘双板带。Lubricin可能通过防止细胞粘附以及在正常双板区功能期间提供润滑而在正常椎间盘后部附着生理学中起作用。(C)2011年Elsevier GmbH。All rights reserved.
Lubricin, which is a boundary joint lubricant, was investigated immunohistochemically in the bilaminar zone (BZ) of the human temporomandibular joint (TMJ), without any degenerative changes. Immunohistochemistry for lubricin detection was carried out on 33 TMJ discs obtained from 17 cadavers. Sections were incubated with diluted rabbit polyclonal anti-lubricin antibody and scored according to the percentage of lubricin immunopositive cells. Three different TMJ disc tissue compartments were analyzed, namely: the upper lamina, the inferior lamina and the loose connective tissue in the space between the laminae. The Mann-Whitney U test was used to compare protein expression (lubricin) among disc specimens' regions. Staining was noted within the TMJ disc cell populations in every disc tissue sample, with almost every cell immunolabeled by the lubricin antibody. The number of disc cells immunolabeled was almost the same in the 3 bilaminar zone regions. Positive extracellular matrix staining was also seen. The results of the present study suggest that lubricin is expressed in the TMJ disc bilaminar zone. Lubricin may have a role in normal disc posterior attachment physiology through the prevention of cellular adhesion as well as providing lubrication during normal bilaminar zone function. (C) 2011 Elsevier GmbH. All rights reserved.