Specific differences in gene expression profile revealed by cDNA microarray analysis of glutathione S-transferase placental form (GST-P) immunohistochemically positive rat liver foci and surrounding tissue.
Specific differences in gene expression profile revealed by cDNA microarray analysis of glutathione S-transferase placental form (GST-P) immunohistochemically positive rat liver foci and surrounding tissue.
复制标题
通过对谷胱甘肽 S-转移酶胎盘型 (GST-P) 免疫组织化学阳性大鼠肝脏病灶和周围组织的 cDNA 微阵列分析揭示基因表达谱的具体差异。
作者：
Shugo Suzuki;M. Asamoto;K. Tsujimura;T. Shirai
胎盘型谷胱甘肽S-转移酶（GST-P）是谷胱甘肽S-转移酶家族中的一种解毒酶，是一种非常有用的大鼠肝脏癌前病变的标志物。我们在这里调查的基因表达谱中GST-P阳性灶相比，周围GST-P阴性地区在同一肝的大鼠治疗二乙基亚硝胺，然后2-乙酰氨基芴结合部分肝切除术。通过激光显微切割收获GST-P阳性病灶，并提取总RNA以允许通过cDNA微阵列测定来评估基因表达谱。发现转醛醇酶、大鼠黄曲霉毒素B1醛还原酶和γ-谷氨酰半胱氨酸合成酶为上调基因，regucalcin为下调基因，与肝细胞癌的发现一致。结果表明，所采用的方法是有用的理解肝癌的发生机制和识别新的标记物为大鼠肝脏癌前病变。
Glutathione S-transferase placental form (GST-P), one of the glutathione S-transferases family of detoxification enzymes, is a very useful marker of rat liver pre-neoplastic lesions. We here investigated the gene expression profile in GST-P positive foci as compared with surrounding GST-P negative areas in the same liver of rats treated with diethylnitrosamine and then 2-acetylaminofluorene combined with partial hepatectomy. GST-P positive foci were harvested by laser microdissection and total RNAs were extracted to allow gene expression profiles to be assessed by cDNA microarray assays. Transaldolase, rat aflatoxin B1 aldehyde reductase and gamma-glutamylcysteine synthetase were found as up-regulated genes and regucalcin as a down-regulated gene, in line with findings for hepatocellular carcinomas. The results indicate that the approach adopted is useful for understanding mechanisms of hepatocarcinogenesis and identification of new markers for rat liver pre-neoplastic foci.
登录
查看更多内容
DOI：
10.1016/s0021-9258(19)36569-x
发表时间：
1993-09
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
C. Huang;Long-Sen Chang;Mary E. Anderson;A. Meister
通讯作者：
C. Huang;Long-Sen Chang;Mary E. Anderson;A. Meister
DOI：
10.1073/pnas.89.7.3070
发表时间：
1992-04-01
影响因子：
11.1
作者：
GODWIN, AK;MEISTER, A;ANDERSON, ME
通讯作者：
ANDERSON, ME
大鼠肾γ-谷氨酰半胱氨酸合成酶的氨基酸序列。
DOI：
--
发表时间：
1990
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Yan,N;Meister,A
通讯作者：
Meister,A
在依托泊苷诱导的氧化应激过程中，高谷胱甘肽和γ-谷氨酰半胱氨酸合成酶水平可保护急性髓细胞白血病细胞免受细胞凋亡。
DOI：
10.1023/a:1008382912096
发表时间：
1999
期刊：
Annals of oncology : official journal of the European Society for Medical Oncology
影响因子：
--
作者：
Siitonen,T;Alaruikka,P;Mäntymaa,P;Savolainen,ER;Kavanagh,TJ;Krejsa,CM;Franklin,CC;Kinnula,V;Koistinen,P
通讯作者：
Koistinen,P