Assessment of ectomycorrhizal biodiversity in Tuber macrosporum productive sites
Assessment of ectomycorrhizal biodiversity in Tuber macrosporum productive sites
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DOI：
10.1007/s00572-013-0538-3
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发表时间：
2014-05-01
期刊：
影响因子：
3.9
通讯作者：
Donnini, Domizia
中科院分区：
文献类型：
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作者：
Benucci, Gian Maria Niccolo;Raggi, Lorenzo;Donnini, Domizia
块茎大孢子 Vittad。是一种具有卓越感官特性的松露，其种植仍处于起步阶段。我们首次致力于提供有关天然和栽培大孢子菌场所的外生菌根群落的信息。使用 ITS nrDNA 测序鉴定外生菌根形态类型，并将其分类为分子操作分类单位 (MOTU)。我们在大孢子菌人工种植园中检测到了 16 个 MOTU。子囊菌门最丰富（86.4%），其中 Helvellaceae、Pyronemataceae 和 Pezizaceae 最常见。在天然大孢子菌位点收集了 22 个 MOTU。担子菌门形态类型丰富(70.6%)，以Thelephoraceae为主。每个地点的地下都有不同的类群，只有大孢子菌是共同的，自然地区 (18.2%) 比栽培地区 (14.4%) 更丰富。栽培地的物种丰富度、辛普森和香农多样性指数、分类多样性、独特性和分类独特性变异均低于自然地。
Tuber macrosporum Vittad. is a truffle with superb organoleptic properties, whose cultivation is still in its infancy. For the first time we have aimed to provide information on ectomycorrhizal communities in natural and cultivated T. macrosporum sites. Ectomycorrhizal morphotypes were identified using ITS nrDNA sequencing and sorted into molecular operational taxonomic unit (MOTU). We detected 16 MOTUs in the T. macrosporum cultivated plantation. Ascomycota were the most abundant (86.4 %) with Helvellaceae, Pyronemataceae and Pezizaceae the most common. Twenty-two MOTUs were collected in the natural T. macrosporum site. Basidiomycota morphotypes were plentiful (70.6 %) and Thelephoraceae dominated. Each site had different taxa belowground with only T. macrosporum in common, being more abundant in the natural (18.2 %) than in the cultivated (14.4 %) site. Species richness, Simpson and Shannon diversity indices, taxonomic diversity, distinctness and variation of taxonomic distinctness were lower in the cultivated than in the natural site.