Initiation of Acquired Immunity in the Lungs of Mice Lacking Lymph Nodes after Infection with Aerosolized Mycobacterium tuberculosis
Initiation of Acquired Immunity in the Lungs of Mice Lacking Lymph Nodes after Infection with Aerosolized Mycobacterium tuberculosis
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DOI：
10.2353/ajpath.2010.090446
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发表时间：
2010-01-01
影响因子：
6
通讯作者：
Campos-Neto, Antonio
中科院分区：
文献类型：
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作者：
Kashino, Suely S.;Vallerskog, Therese;Campos-Neto, Antonio
最近的证据表明，肺引流淋巴结是雾化结核分枝杆菌感染小鼠启动免疫反应的部位。在这里，我们扩展了这些研究，并表明，雾化结核分枝杆菌感染缺乏淋巴结的小鼠，在感染后14天内会导致大量单核细胞渗入肺部。这种浸润明显类似于支气管相关淋巴组织的扩张。正如预期的那样，在感染结核分枝杆菌的野生型对照小鼠中没有观察到与支气管相关的淋巴组织。重要的是，早在感染后14天，从没有淋巴结的小鼠的肺淋巴细胞中就可以检测到对结核分枝杆菌抗原的获得性特异性免疫反应。此外，这些小鼠的细菌负荷与野生型C57BL/6对照组小鼠的细菌负荷没有什么区别。这些结果表明，在没有淋巴结的情况下，雾化结核分枝杆菌感染小鼠后，免疫反应的启动发生在肺组织中，清楚地说明了免疫系统对外来病原体产生抵抗力的巨大可塑性。(Am J Pathol2010,176198-204DOI：10.2353/ajpath.2010.090446)
Recent evidence points to lung draining lymph nodes as the site that initiates the immune response in mice infected with aerosolized Mycobacterium tuberculosis. Here we expanded these studies and showed that infection of mice that lack lymph nodes with aerosolized M. tuberculosis results in a massive mononuclear cell infiltrate in the lungs within 14 days postinfection. This infiltration clearly resembles an expansion of the bronchus-associated lymphoid tissue. As expected, no bronchus-associated lymphoid tissue was observed in M. tuberculosis-infected wild-type control mice. Importantly, acquired specific immune response to M. tuberculosis antigens could be detected in lung lymphocytes harvested from mice lacking lymph nodes as early as 14 days postinfection. In addition, the bacterial burden in these mice was indistinguishable from that observed in wild-type C57BL/6 control mice. These results indicate that in the absence of lymph nodes, priming of the immune response occurs in the lung tissues after infection of mice with aerosolized M. tuberculosis and clearly illustrate the enormous plasticity of the immune system to develop resistance to foreign pathogens. (Am J Pathol 2010, 176:198-204; DOI: 10.2353/ajpath.2010.090446)