EXPRESSION OF RECOMBINANT GENES IN MYOCARDIUM INVIVO AFTER DIRECT INJECTION OF DNA
EXPRESSION OF RECOMBINANT GENES IN MYOCARDIUM INVIVO AFTER DIRECT INJECTION OF DNA
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DOI：
10.1161/01.cir.82.6.2217
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发表时间：
1990-12-01
期刊：
影响因子：
37.8
通讯作者：
LEIDEN, JM
中科院分区：
文献类型：
--
作者：
LIN, H;PARMACEK, MS;LEIDEN, JM
在体内心肌细胞中编程重组基因表达的能力为许多遗传性和获得性心血管疾病的治疗带来了希望。在本报告中，我们证明了重组β-在劳斯肉瘤病毒启动子控制下的半乳糖苷酶基因可以通过将纯化的质粒DNA直接注射到左心室壁中而在体内导入成年大鼠心肌细胞中并在其中表达。表达重组β-受体的心肌细胞在注射β-半乳糖苷酶后至少4周，在大鼠心脏中进行组织化学检测。半乳糖苷酶基因这些结果证明了这种体细胞基因治疗方法用于治疗心血管疾病的潜力。
The ability to program recombinant gene expression in cardiac myocytes in vivo holds promise for the treatment of many inherited and acquired cardiovascular diseases. In this report, we demonstrate that a recombinant .beta.-galactosidase gene under the control of the Rous sarcoma virus promoter can be introduced into and expressed in adult rat cardiac myocytes in vivo by the injection of purified plasmid DNA directly into the left ventricular wall. Cardiac myocytes expressing recombinant .beta.-galactosidase were detected histochemically in rat hearts for at least 4 weeks after injection of the .beta.-galactosidase gene. These results demonstrate the potential of this method of somatic gene therapy for the treatment of cardiovascular disease.