Lack of effect of butyrate on S phase DNA synthesis despite presence of histone hyperacetylation.
Lack of effect of butyrate on S phase DNA synthesis despite presence of histone hyperacetylation.
复制标题
尽管存在组蛋白过度乙酰化，但丁酸盐对 S 期 DNA 合成缺乏影响。
DOI：
10.1016/0014-4827(82)90216-6
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发表时间：
1982
影响因子：
3.7
通讯作者：
Cidlowski,JA
中科院分区：
文献类型：
--
作者：
Littlefield,BA;Cidlowski,NB;Cidlowski,JA
The effects of sodium butyrate on [3H]thymidine incorporation and cell growth characteristics in randomly growing and synchronized HeLa S3cells have been examined in an attempt to determine what effects, if any, butyrate has on S phase cells. Whereas 5 mM sodium butyrate rapidly inhibits [5H]thymidine incorporation in a randomly growing cell populations, it has no effect on incorporation during the S phase in cells synchronized by double thymidine block techniques. This lack of effect does not result from an impaired ability of the S phase cells to take up butyrate, since butyrate administration during this period leads to histone hyperacetylation that is identical with that seen with butyrate treatment of randomly growing cells. Furthermore, the ability to induce such hyperacetylation with butyrate during an apparently normal progression through S phase indicates that histone hyperacetylation probably has no effect on the overall process of DNA replication. Temporal patterns of [3H]thymidine incorporation and cell growth following release from a 24-h exposure to butyrate confirm blockage of cell growth in the G1 phase of the cell cycle. Thus, the inhibition by butyrate of [3H]thymidine incorporation in randomly growing HeLa S3cell populations can be accounted for solely on the basis of a G1 phase block, with no inhibitory effects on cells already engaged in DNA synthesis or cells beyond the G1 phase block at the time of butyrate administration.
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