Single-Cell RNA Sequencing (scRNA-seq) in Cardiac Tissue: Applications and Limitations.
Single-Cell RNA Sequencing (scRNA-seq) in Cardiac Tissue: Applications and Limitations.
复制标题
心脏组织中的单细胞RNA测序（SCRNA-SEQ）：应用和局限性。
DOI：
10.2147/vhrm.s288090
复制
发表时间：
2021
影响因子：
2.9
通讯作者：
Tian L
中科院分区：
文献类型：
--
作者：
Wang M;Gu M;Liu L;Liu Y;Tian L
心血管疾病(CVD)是一组心血管和心脏疾病，被认为是世界范围内主要的死亡原因。心血管疾病的病理可能与心脏中多种细胞类型的功能异常有关。单细胞RNA测序(scRNA-seq)技术通过在单细胞水平上提供高分辨率的转录变化，是鉴定单个细胞和阐明其分子机制的一种强有力的方法。特别是，scRNA-seq通过识别罕见的心脏细胞类型、推断轨迹树、估计RNA速度、阐明细胞间的通讯以及比较健康和病理心脏样本，为心血管疾病提供了新的见解。在这篇综述中，我们总结了心血管领域中不同的scRNA-seq平台和已发表的单细胞数据集，并描述了这项技术的实用性和局限性。最后，对scRNA-seq技术在心血管研究中的应用前景进行了展望。
Cardiovascular diseases (CVDs) are a group of disorders of the blood vessels and heart, which are considered as the leading causes of death worldwide. The pathology of CVDs could be related to the functional abnormalities of multiple cell types in the heart. Single-cell RNA sequencing (scRNA-seq) technology is a powerful method for characterizing individual cells and elucidating the molecular mechanisms by providing a high resolution of transcriptomic changes at the single-cell level. Specifically, scRNA-seq has provided novel insights into CVDs by identifying rare cardiac cell types, inferring the trajectory tree, estimating RNA velocity, elucidating the cell–cell communication, and comparing healthy and pathological heart samples. In this review, we summarize the different scRNA-seq platforms and published single-cell datasets in the cardiovascular field, and describe the utilities and limitations of this technology. Lastly, we discuss the future perspective of the application of scRNA-seq technology into cardiovascular research.