Finite difference time domain modeling of wavefront aberrations in bone using second harmonic generation microscopy
Finite difference time domain modeling of wavefront aberrations in bone using second harmonic generation microscopy
复制标题
使用二次谐波发生显微镜对骨中波前像差进行有限差分时域建模
DOI：
10.1117/12.2290376
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发表时间：
2018
期刊：
影响因子：
--
通讯作者：
Mortensen, Luke J.
中科院分区：
文献类型：
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作者：
Tehrani, Kayvan F.;Phang, Sendy;Kner, Peter;Vukovic, Ana;Mortensen, Luke J.
近红外和红外多光子骨成像是一个新兴的领域，有望帮助回答许多需要微创活体成像的生物学问题。神经科学研究人员尤其开始利用长波成像来克服多次散射，并通过完整或部分完整的骨骼成像大脑深处。由于小鼠模型在许多生物学实验中都被使用，我们在这里研究了小鼠颅骨引起的光学像差，以及它们对光传播的影响。我们以前开发了一种光线跟踪模型，该模型利用骨胶原纤维中的二次谐波来估计样品的折射率结构。该技术能够为闭环自适应光学系统快速提供初始信息。但是，光线跟踪方法不考虑折射或散射。在这里，我们扩展了我们的工作，使用一个完整的电磁模型来研究骨中的波前像差。在二次谐波成像获得的折射率骨数据集上，我们使用了光传播的有限差分时间域模型。在这篇文章中，我们展示了视场中不同起始点的模型化波前相位。
Near infrared and infrared multi-photon imaging through or inside bone is an emerging field that promises to help answer many biological questions that require minimally invasive intravital imaging. Neuroscience researchers especially have begun to take advantage of long wavelength imaging to overcome multiple scattering and image deep inside the brain through intact or partially intact bone. Since the murine model is used in many biological experiments, here we investigate the optical aberrations caused by mouse cranial bone, and their effects on light propagation. We previously developed a ray tracing model that uses second harmonic generation in collagen fibers of bone to estimate the refractive index structure of the sample. This technique is able to rapidly provide initial information for a closed loop adaptive optics system. However, the ray tracing method does not account for refraction or scattering. Here, we extend our work to investigate the wavefront aberrations in bone using a full electromagnetic model. We used Finite-Difference Time-Domain modeling of light propagation in refractive index bone datasets acquired with second harmonic generation imaging. In this paper we show modeled wavefront phase from different originating points across the field of view.