Purification, crystallization and preliminary X-ray analysis of two hydrogen sulfide-producing enzymes from Fusobacterium nucleatum
Purification, crystallization and preliminary X-ray analysis of two hydrogen sulfide-producing enzymes from Fusobacterium nucleatum
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具核梭杆菌两种产硫化氢酶的纯化、结晶和初步 X 射线分析
DOI：
10.1107/s1744309112042546
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发表时间：
2012
期刊：
影响因子：
--
通讯作者：
Nonaka
中科院分区：
文献类型：
--
作者：
Kezuka Y;Abe N;Yoshida Y;Nonaka
口腔细菌产生的硫化氢是造成口腔异味的原因。从核梭杆菌（积极产生硫化氢）中过量生产两个同源的硫化氢产生酶Fn1220和Cdl，纯化和结晶。利用同步辐射源从晶体中收集x射线衍射数据。Fn1220晶体属于四方空间群P41212或P43212（单位胞参数a = b = 116.8, c = 99.2 Å）， Cdl晶体属于单斜空间群P21（单位胞参数a = 84.9, b = 70.9, c = 87.6 Å， β = 90.3°）。
Hydrogen sulfide produced by oral bacteria is responsible for oral malodour. Two homologous hydrogen sulfide-producing enzymes, Fn1220 and Cdl, from Fusobacterium nucleatum (which actively produces hydrogen sulfide) were overproduced, purified and crystallized. X-ray diffraction data were collected from the crystals using a synchrotron-radiation source. The Fn1220 crystal belonged to tetragonal space group P41212 or P43212 (unit-cell parameters a = b = 116.8, c = 99.2 Å) and the Cdl crystal belonged to monoclinic space group P21 (unit-cell parameters a = 84.9, b = 70.9, c = 87.6 Å, β = 90.3°).