Spectroscopic tags using dye-embedded nanoparticles and surface-enhanced Raman scattering
Spectroscopic tags using dye-embedded nanoparticles and surface-enhanced Raman scattering
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DOI：
10.1021/ac034672u
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发表时间：
2003-11-15
影响因子：
7.4
通讯作者：
Nie, SM
中科院分区：
文献类型：
--
作者：
Doering, WE;Nie, SM
表面增强拉曼散射能够在单分子和单纳米颗粒水平上提供丰富的振动信息，但这种巨大的增强效应的实际应用仍然是一个挑战。在这里，我们报告了一类新的染料嵌入的核壳纳米粒子，是非常有效的表面拉曼增强，并可用作光谱标签的多路检测和光谱。核-壳颗粒含有用于光学增强的金属核、用于光谱特征的报告分子和用于保护和缀合的包封二氧化硅壳。令人惊讶的发现是，具有异硫氰酸酯（-N=C=S）基团或多个硫原子的有机分子与二氧化硅包封相容。与荧光染料和量子点相比，增强型拉曼探针包含用于信号放大的内置机制，并且在环境实验条件下提供丰富的光谱信息。
Surface-enhanced Raman scattering is capable of providing rich vibrational information at the level of single molecules and single nanoparticles, but the practical applications of this enormous enhancement effect are still a challenge. Here we report a new class of dye-embedded core-shell nanoparticles that are highly efficient for surface Raman enhancement and could be used as spectroscopic tags for multiplexed detection and spectroscopy. The core-shell particles contain a metallic core for optical enhancement, a reporter molecule for spectroscopic signature, and an encapsulating silica shell for protection and conjugation. A surprising finding is that organic molecules with an isothiocyanate (-N=C=S) group or multiple sulfur atoms are compatible with silica encapsulation. In comparison with fluorescent dyes and quantum dots, enhanced Raman probes contain a built-in mechanism for signal amplification and provide rich spectroscopic information under ambient experimental conditions.