THE MOLECULAR-BASIS OF P-M HYBRID DYSGENESIS - THE ROLE OF THE P-ELEMENT, A P-STRAIN-SPECIFIC TRANSPOSON FAMILY
THE MOLECULAR-BASIS OF P-M HYBRID DYSGENESIS - THE ROLE OF THE P-ELEMENT, A P-STRAIN-SPECIFIC TRANSPOSON FAMILY
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DOI：
10.1016/0092-8674(82)90463-9
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发表时间：
1982-01-01
期刊：
影响因子：
64.5
通讯作者：
RUBIN, GM
中科院分区：
文献类型：
--
作者：
BINGHAM, PM;KIDWELL, MG;RUBIN, GM
我们之前已经证明，在PM基因异常杂交种中产生的5个白色突变等位基因中，有4个是由于插入了高度同源的DNA序列元件而产生的。我们把这些元素命名为P元素。我们报告说，P元素存在于所有P菌株每个单倍体基因组的3040个拷贝中，而在所有M菌株中，P元素显然完全缺失，只有一个例外。此外，P家族成员在PM基因异常杂交种中明显地频繁转座；PM基因异常杂交种的后代染色体中经常出现新获得的P元素。最后，PM基因异常杂交种中x2 PX染色体重排的菌株特异性断点热点可能是P元素的驻留位点。这些观察结果有力地支持了P因子假说，即PM杂交发育不良的机制基础。
We have shown previously that four of five white mutant alleles arising in PM dysgenic hybrids result from the insertion of strongly homologous DNA sequence elements. We have named these P elements. We report that P elements are present in 3040 copies per haploid genome in all P strains examined and apparently are missing entirely from all M strains examined, with one exception. Furthermore, members of the P family apparently transpose frequently in PM dysgenic hybrids; chromosomes descendant from PM dysgenic hybrids frequently show newly acquired P elements. Finally, the strain-specific breakpoint hotspots for the rearrangement of the x2 PX chromsome occurring in PM dysgenic hybrids are apparently sites of residence of P elements. These observations strongly support the P factor hypothesis for the mechanistic basis of PM hybrid dysgenesis.