Molecular pathogenesis of Listeria monocytogenes in the alternative model host Galleria mellonella
Molecular pathogenesis of Listeria monocytogenes in the alternative model host Galleria mellonella
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DOI：
10.1099/mic.0.040782-0
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发表时间：
2010-11-01
期刊：
影响因子：
2.8
通讯作者：
Gahan, Cormac G. M.
中科院分区：
文献类型：
--
作者：
Joyce, Susan A.;Gahan, Cormac G. M.
大蜡螟的幼虫为许多人类病原体提供了另一种感染模型，因为它们适合在较高的孵化温度（37 摄氏度）下使用。这项研究和 Mukherjee 等人的并行研究。 [Mukherjee, K.、Altincek, B.、Hain, T.、Domann, E.、Vilcinskas, A. 和 Chakraborty, T. (2010)。 Appl Environ Microbiol 76, 310-317] 将此昆虫宿主建立为研究李斯特氏菌发病机制的适当模型。在这项研究中，我们表明，接种单增李斯特菌会引发大蜡螟的动态感染，并且溶细胞素李斯特菌溶血素 O (LLO) 的产生对于毒性和细菌生长是必要的。非致病性乳酸乳球菌产生的 LLO 足以诱导昆虫模型死亡。我们采用实时生物发光成像来检查大蜡螟模型中李斯特菌生长和毒力基因表达的动态。对 lux 启动子融合的分析表明，在 30 和 37 摄氏度下将病原体引入昆虫体内后，毒力基因表达显着诱导。检查了宿主对李斯特菌感染的反应，证明血细胞破坏伴随着单核细胞增生李斯特菌的发病机制，并且先于酚氧化酶系统的激活。此外，我们证明无害李斯特菌通过持久性机制对大蜡蚧具有致病性，这暗示了该模型中致病性的替代机制。
Larvae of Galleria mellonella, the greater wax moth, provide an alternative infection model for many human pathogens as they are amenable to use at elevated incubation temperatures (37 degrees C). This study and a parallel study by Mukherjee et al. [Mukherjee, K., Altincicek, B., Hain, T., Domann, E., Vilcinskas, A. & Chakraborty, T. (2010). Appl Environ Microbiol 76, 310-317] establish this insect host as an appropriate model to investigate the pathogenesis of Listeria species. In this study we show that inoculation with Listeria monocytogenes initiates a dynamic infection in G. mellonella and that production of the cytolysin listeriolysin O (LLO) is necessary for toxicity and bacterial growth. Production of LLO by the non-pathogenic species Lactococcus lactis is sufficient to induce mortality in the insect model. We employed real-time bioluminescence imaging to examine the dynamics of listerial growth and virulence gene expression in the G. mellonella model. Analysis of lux promoter fusions demonstrated significant induction of virulence gene expression upon introduction of the pathogen into insects at both 30 and 37 degrees C. The host response to listerial infection was examined which demonstrated that haemocyte destruction accompanies L. monocytogenes pathogenesis and is preceded by activation of the phenoloxidase system. Furthermore, we demonstrate that Listeria innocua is pathogenic to G. mellonella through a persistence mechanism that implicates an alternative mechanism for pathogenicity in this model.