Relative survival of Bacillus subtilis spores loaded on filtering facepiece respirators after five decontamination methods
Relative survival of Bacillus subtilis spores loaded on filtering facepiece respirators after five decontamination methods
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DOI：
10.1111/ina.12475
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发表时间：
2018-09-01
期刊：
影响因子：
5.8
通讯作者：
Lai, C. -Y.
中科院分区：
文献类型：
--
作者：
Lin, T. -H.;Tang, F. -C.;Lai, C. -Y.
本研究测定了N95过滤式面罩（FFR）上枯草芽孢杆菌（Bacillus subtilis）孢子经5种方法净化后在最坏条件下的相对存活率（RS）。在去污和37℃、95%相对湿度保存24小时后，检测ffr的相对存活率。除污方法包括乙醇、漂白剂、紫外线照射（UVA 365nm、UVC 254nm）、高压灭菌器和台湾制造的传统电饭煲（TERC）。在不消毒的情况下，59 +/- 8%的负载孢子存活了24小时。当N95 FFR中添加70%乙醇，包装密度为0.23时，初始RS为73 +/- 5%，24h后衰减为22 +/- 8%。UVA照射20分钟后，相对存活率保持在20%以上。其他四种去污措施在对试验ffr应用后立即测量出99%-100%的杀生物效果。相对存活期是衡量灭菌或消毒程度的有用参数。漂白剂、UVC、高压灭菌器和TERC比乙醇和UVA具有更好的杀菌效果。较高的过滤质量和较低的RS值都能产生最净化的FFR。
This study determines the relative survival (RS) of Bacillus subtilis spores loaded on an N95 filtering facepiece respirator (FFR) after decontamination by five methods under worst-case conditions. Relative survival was obtained by testing after decontamination and after storing the FFRs at 37 degrees C and 95% relative humidity for 24hours. The decontamination methods involved ethanol, bleach, ultraviolet irradiation (UVA 365nm, UVC 254nm), an autoclave, and a traditional electric rice cooker (TERC) that was made in Taiwan. Without decontamination, 59 +/- 8% of the loaded spores survived for 24hours. When 70% ethanol was added to the N95 FFR at a packing density of 0.23, the RS was 73 +/- 5% initially and decayed to 22 +/- 8% in 24hours. Relative survival remained above 20% after 20minutes of UVA irradiation. The other four decontamination measures achieved 99%-100% biocidal efficacy, as measured immediately after the methods were applied to the test FFRs. Relative survival is a useful parameter for measuring sterilization or degree of disinfection. Bleach, UVC, an autoclave, and a TERC provide better biocidal efficacy than ethanol and UVA. Not only a higher filter quality but also a lower value of RS produced the most decontaminated FFR.