Increased expression of cytokines, soluble cytokine receptors, soluble apoptosis ligand and apoptosis in dengue
Increased expression of cytokines, soluble cytokine receptors, soluble apoptosis ligand and apoptosis in dengue
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DOI：
10.1016/j.virol.2013.12.027
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发表时间：
2014-03-01
期刊：
影响因子：
3.7
通讯作者：
Alvarez-Mon, Melchor
中科院分区：
文献类型：
--
作者：
Arias, Julia;Valero, Nereida;Alvarez-Mon, Melchor
已经进行了几项研究，以确定生物标志物，这些生物标志物定义了发展严重形式登革热的风险因素。在这项研究中，TNF-α，IL-6，IL-1，IL-17，可溶性白细胞介素-1受体样1蛋白（sST 2），可溶性TNF相关凋亡诱导配体（sTRAIL），IL-12和可溶性TNF受体（sTNF-RI和sTNF-RII）的水平通过ELISA在登革热患者和单核细胞/巨噬细胞培养物中测定。登革热分为无警告症状登革热（DNWS）、有警告症状登革热（DWWS）和严重登革热（SD）。在DWWS和/或SD中观察到高值的IL-6、sTNFRI、sTNFRII和sST 2，在DNWS中观察到高值的IL-12和sTRAIL。TNF-α和IL-17升高与疾病严重程度无关。在登革单核细胞/巨噬细胞培养物中观察到TNF-α、IL-1 β、IL-12、IL-17、sST 2和sTRAIL的高产量和凋亡表达。这项研究表明，无论疾病的严重程度如何，有益或有害的生物标志物都可能存在于登革热中，单核细胞可能是所研究分子的部分来源。(C)2014爱思唯尔公司All rights reserved.
Several studies have been performed to determine biomarkers that define the risk factors to developing severe forms of dengue. In this study, the levels of TNF-alpha, IL-6, IL-1, IL-17, soluble interleukin-1 receptor like 1 protein (sST2), soluble TNF-related apoptosis-inducing ligand (sTRAIL), IL-12 and soluble receptors for TNF (sTNF-RI and sTNF-RII) were determined by ELISA in dengue patients and monocyte/macrophage cultures. Dengue was classified as dengue without warning symptoms (DNWS), with warning symptoms (DWWS) and severe dengue (SD). High values of IL-6, sTNFRI, sTNFRII and sST2 were observed in DWWS and/or SD and IL-12 and sTRAIL in DNWS. TNF-alpha and IL-17 were increased not associated to the disease severity. High production of TNF-alpha, IL-1 beta, IL-12, IL-17, sST2 and sTRAIL and apoptosis expression were observed in dengue monocyte/macrophage cultures. This study shows that beneficial or deleterious biomarkers can be present in dengue regardless the disease severity and that monocytes may be in part the source of studied molecules. (C) 2014 Elsevier Inc. All rights reserved.