Differential production of IL-2 and IL-4 mRNA in vivo after primary sensitization.
Differential production of IL-2 and IL-4 mRNA in vivo after primary sensitization.
复制标题
初次致敏后体内 IL-2 和 IL-4 mRNA 的差异产生。
作者：
K. Mohler;L. Butler
淋巴因子的研究几乎完全是利用体外检测系统、长期细胞系和克隆进行的。因此，关于特定抗原刺激后在体内产生淋巴因子/细胞因子的信息很少。为了解决这一局限性，我们修改了mRNA表型系统，以允许在体内抗原刺激后对淋巴因子mRNA进行定量。在此，我们报道了苦味氯致敏后体内IL-2和IL-4mRNA的产生。然而，IL-2和IL-4mRNA产生的时间过程是不一致的。IL-2m RNA的表达主要发生在抗原暴露后1~3d，而IL-4m RNA的表达主要发生在3d至5d。因此，苦味酸致敏后，这两种淋巴因子m RNA在体内的产生呈现“级联”现象。这些结果1)表明IL-4mRNA是在体内的初级免疫反应中诱导的，2)增加了体内免疫反应的产生可能涉及特定淋巴因子的特定顺序产生。
The study of lymphokines has been almost entirely conducted by utilizing in vitro assay systems, long term cell lines, and clones. Thus, little information is available concerning the production of lymphokines/cytokines in vivo after specific antigenic stimulation. In order to address this limitation, we have modified the mRNA phenotyping system to allow for the quantitation of lymphokine mRNA after antigenic stimulation in vivo. We report here the production of both IL-2 and IL-4 mRNA in vivo after primary sensitization with picryl chloride. However, the time course of IL-2 and IL-4 mRNA production is discordant. The majority of IL-2 mRNA expression occurs from 1 to 3 days after antigenic exposure, whereas IL-4 mRNA expression occurs mainly from day 3 through day 5. Thus, the production in vivo of these two lymphokine mRNA after sensitization with picryl chloride appears to occur as a "cascade." These results 1) demonstrate that IL-4 mRNA is induced during a primary immune response in vivo and 2) raise the possibility that the generation of an immune response in vivo may involve a specific sequential production of certain lymphokines.